Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry

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dc.contributor.author Day, Jennifer R.
dc.contributor.author Kell, Douglas B.
dc.contributor.author Griffith, Gareth W.
dc.contributor.editor en_US
dc.date.accessioned 2010-01-08T17:48:42Z
dc.date.available 2010-01-08T17:48:42Z
dc.date.issued 2002
dc.identifier.citation Day, J. P., Kell, D. B., Griffith, G. W. (2002). Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry.   Applied and Environmental Microbiology, 68, (1), 37-45. en
dc.identifier.isbn en_US
dc.identifier.issn en_US
dc.identifier.uri http://hdl.handle.net/2160/3959
dc.description.abstract The ability of two different flow cytometers, the Microcyte (Optoflow) and the PAS-III (Partec), to differentiate sporangia of the late-blight pathogen Phytophthora infestans from other potential airborne particles was compared. With the PAS-III, light scatter and intrinsic fluorescence parameters could be used to differentiate sporangia from conidia of Alternaria or Botrytis spp., rust urediniospores, and pollen of grasses and plantain. Differentiation between P. infestans sporangia and powdery mildew conidia was not possible by these two methods but, when combined with analytical rules evolved by genetic programming methods, could be achieved after staining with the fluorescent brightener Calcofluor white M2R. The potential application of these techniques to the prediction of late-blight epiphytotics in the field is discussed. en
dc.language.iso en en
dc.publisher American Society for Microbiology en
dc.relation.ispartof en_US
dc.relation.isreferencedby http://dx.doi.org/10.1128/AEM.68.1.37-45.2002 en
dc.title Differentiation of Phytophthora infestans sporangia from other airborne biological particles by flow cytometry en
dc.title.alternative en_US
dc.type Text en
dc.type.publicationtype refereed published journal paper en
dc.identifier.duplicate True


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