Show simple item record Mochizuki, Takashi Tanabe, Hiroshi Kawasaki, Masako Jackson, Colin J. Ishizaki, Hiroshi 2010-01-10T12:24:38Z 2010-01-10T12:24:38Z 2003-06-01
dc.identifier.citation Mochizuki , T , Tanabe , H , Kawasaki , M , Jackson , C J & Ishizaki , H 2003 , ' Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions ' Journal of Dermatological Science , vol 32 , no. 1 , pp. 25-32 . DOI: 10.1016/S0923-1811(03)00030-6 en
dc.identifier.issn 0923-1811
dc.identifier.other PURE: 138368
dc.identifier.other PURE UUID: 695a73cd-4e11-48da-8818-9c200f4325ac
dc.identifier.other dspace: 2160/3966
dc.identifier.other DSpace_20121128.csv: row: 3157
dc.identifier.other Scopus: 0037533693
dc.description Mochizuki, T., Ranabe, H., Kawasaki, M., Ishizaki, H., Jackson, C. J. (2003). Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions.   Journal of Dermatological Science, Elsevier, 32, (1), 25-32 en
dc.description.abstract Background: Culture morphology of Trichophyton (T.) tonsurans, an emerging pathogen of dermatophytosis in Japan, varies widely and species level identification is sometimes very difficult. Reliable molecular markers are expected to be introduced for their identification. Objective: The present study was conducted to evaluate the efficacy of restriction fragment length polymorphism (RFLP) analysis of PCR amplified ribosomal (r) DNA including internal transcribed spacers (ITS), as an identification tool. Methods: Total cellular DNA was extracted from 26 Japanese isolates of T. tonsurans, along with several taxa of the members in the T. mentagrophytes complex, T. rubrum, T. violaceum and Epidermophyton floccosum, using a mini-preparation method. PCR amplicons were digested with restriction enzymes Mva I or Hinf I, then electrophoresed on 5% polyacrylamide gel. Results: The banding profiles were observed about 8 h from initiating DNA extraction. Intraspecies polymorphism was not detected among T. tonsurans isolates, and their profiles obtained using Mva I digestion were clearly different from those of the other dermatophyte species. The restriction profiles evaluated from nucleotide sequence of the regions by a computer analysis were compatible with the electrophoresed profiles on gel. Conclusion: PCR-RFLP analysis is a rapid and reliable tool for the identification of T. tonsurans. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Journal of Dermatological Science en
dc.rights en
dc.subject PCR-RFLP analysis en
dc.subject indentification en
dc.subject dermatophytes en
dc.subject ribosomal DNA en
dc.subject restriction enzyme analysis en
dc.subject Trichophyton tonsurans en
dc.title Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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