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dc.contributor.author Jackson, Colin J.
dc.contributor.author Ishizaki, Hiroshi
dc.contributor.author Mochizuki, T.
dc.contributor.author Kawasaki, M.
dc.contributor.author Ranabe, H.
dc.date.accessioned 2010-01-10T12:24:38Z
dc.date.available 2010-01-10T12:24:38Z
dc.date.issued 2003-06
dc.identifier.citation Jackson , C J , Ishizaki , H , Mochizuki , T , Kawasaki , M & Ranabe , H 2003 , ' Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions ' Journal of Dermatological Science , vol 32 , no. 1 , pp. 25-32 . , 10.1016/S0923-1811(03)00030-6 en
dc.identifier.issn 0923-1811
dc.identifier.other PURE: 138368
dc.identifier.other dspace: 2160/3966
dc.identifier.uri http://hdl.handle.net/2160/3966
dc.description Mochizuki, T., Ranabe, H., Kawasaki, M., Ishizaki, H., Jackson, C. J. (2003). Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions.   Journal of Dermatological Science, Elsevier, 32, (1), 25-32 en
dc.description.abstract Background: Culture morphology of Trichophyton (T.) tonsurans, an emerging pathogen of dermatophytosis in Japan, varies widely and species level identification is sometimes very difficult. Reliable molecular markers are expected to be introduced for their identification. Objective: The present study was conducted to evaluate the efficacy of restriction fragment length polymorphism (RFLP) analysis of PCR amplified ribosomal (r) DNA including internal transcribed spacers (ITS), as an identification tool. Methods: Total cellular DNA was extracted from 26 Japanese isolates of T. tonsurans, along with several taxa of the members in the T. mentagrophytes complex, T. rubrum, T. violaceum and Epidermophyton floccosum, using a mini-preparation method. PCR amplicons were digested with restriction enzymes Mva I or Hinf I, then electrophoresed on 5% polyacrylamide gel. Results: The banding profiles were observed about 8 h from initiating DNA extraction. Intraspecies polymorphism was not detected among T. tonsurans isolates, and their profiles obtained using Mva I digestion were clearly different from those of the other dermatophyte species. The restriction profiles evaluated from nucleotide sequence of the regions by a computer analysis were compatible with the electrophoresed profiles on gel. Conclusion: PCR-RFLP analysis is a rapid and reliable tool for the identification of T. tonsurans. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Journal of Dermatological Science en
dc.title Rapid identification of Trichophyton tonsurans by PCR-RFLP analysis of ribosomal DNA regions en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/S0923-1811(03)00030-6
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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