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dc.contributor.author Lamb, David C.
dc.contributor.author Manning, Nigel J.
dc.contributor.author Jackson, Colin J.
dc.contributor.author Kelly, Diane E.
dc.contributor.author Kelly, Steven L.
dc.date.accessioned 2010-01-10T12:36:36Z
dc.date.available 2010-01-10T12:36:36Z
dc.date.issued 2003-10-03
dc.identifier.citation Lamb , D C , Manning , N J , Jackson , C J , Kelly , D E & Kelly , S L 2003 , ' Mutations in Saccharomyces cerevisiae sterol C5-desaturase conferring resistance to the CYP51 inhibitor fluconazole ' Biochemical and Biophysical Research Communications , vol 309 , no. 4 , pp. 999-1004 . , 10.1016/j.bbrc.2003.08.098 en
dc.identifier.issn 1090-2104
dc.identifier.other PURE: 138325
dc.identifier.other dspace: 2160/3967
dc.identifier.uri http://hdl.handle.net/2160/3967
dc.description Jackson, C. J., Lamb, D. C., Manning, N. J., Kelly, D. E., Kelly, S. L. (2003). Mutations in Saccharomyces cerevisiae sterol C5-desaturase conferring resistance to the CYP51 inhibitor fluconazole.  Biochemical and Biophysical Research Communications, 309, (4), 999-1004. en
dc.description.abstract Understanding fluconazole resistance is important as it emerged as a serious clinical problem for this CYP51, sterol 14α-demethylase, inhibitor. One mechanism, observed first in Saccharomyces cerevisiae, was through defective sterol C5-desaturase (Erg3p) required to form the fungistatic sterol end-product resulting from CYP51 inhibition, 14α-methylergosta-8,24(28)-dien-3β,6α-diol. Here, we report molecular changes resulting in both blocked mutants and also leaky mutants in which reduced ergosterol levels were detected. Blocked mutants exhibited nonsense and frameshift mutations, while leaky mutants contained missense mutations that were generally in conserved positions based on the alignment of sterol C5-desaturases and located mainly between residues 250 and 282. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Biochemical and Biophysical Research Communications en
dc.subject Sterol C5-desaturase en
dc.subject Cytochrome P450 inhibition en
dc.subject Fluconazole en
dc.subject Antifungal resistance en
dc.subject Mutation en
dc.title Mutations in Saccharomyces cerevisiae sterol C5-desaturase conferring resistance to the CYP51 inhibitor fluconazole en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.bbrc.2003.08.098
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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