Show simple item record T. en_US Gerard J. en_US T. en_US Tsuyoshi en_US Joanne en_US J. B. en_US 2010-01-10T12:39:36Z 2010-01-10T12:39:36Z 2003-10-13 en_US
dc.identifier en_US
dc.identifier.citation Yokota , T , Bishop , G J , Nomura , T , Kaneta , T , Chory , J & Reid , J B 2003 , ' The LKA gene is a BRASSINOSTEROID INSENSITIVE 1 homolog of pea ' Plant Journal , vol 36 , no. 3 , pp. 291-300 . , 10.1046/j.1365-313X.2003.01863.x en_US
dc.identifier.other PURE: 138307 en_US
dc.identifier.other dspace: 2160/3968 en_US
dc.description.abstract Brassinosteroids (BRs) are growth-promoting plant steroid hormones, and in garden pea (Pisum sativum L.), the lka mutant is defective in BR perception. Here, we show that LKA encodes P. sativum BRI1 (PsBRI1), a homolog of BRI1, which is the Arabidopsis leucine-rich repeat receptor-like kinase/BR receptor. PsBRI1 was isolated by screening a pea cDNA library using Arabidopsis BRI1 cDNA as the probe. PsBRI1 is predicted to encode a 1188-amino-acid protein that has 78% similarity with Arabidopsis BRI1. Sequence analysis of PsBRI1 in the lka mutant led to the identification of a missense mutation that converts the highly conserved aspartic acid residue to asparagine, which is located in the leucine-rich repeat, just before the island domain that may bind BR or a BR–protein complex. The mutation identified in PsBRI1 co-segregated with the semi-erectoide lka phenotype. Transcript analysis of LKA/PsBRI1 indicates that it is ubiquitously expressed in pea and that the expression was downregulated by exogenous BR. The lka mutant was then utilized in further studies to analyze the independent actions of BR and gibberellin (GA) through the characterization of BR response on GA mutants and GA response on BR mutants. en_US
dc.format.extent 10 en_US
dc.relation.ispartof Plant Journal en_US
dc.title The LKA gene is a BRASSINOSTEROID INSENSITIVE 1 homolog of pea en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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