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dc.contributor.author Yokota, T.
dc.contributor.author Bishop, Gerard J.
dc.contributor.author Nomura, T.
dc.contributor.author Kaneta, Tsuyoshi
dc.contributor.author Chory, Joanne
dc.contributor.author Reid, J. B.
dc.date.accessioned 2010-01-10T12:39:36Z
dc.date.available 2010-01-10T12:39:36Z
dc.date.issued 2003-10-13
dc.identifier.citation Yokota , T , Bishop , G J , Nomura , T , Kaneta , T , Chory , J & Reid , J B 2003 , ' The LKA gene is a BRASSINOSTEROID INSENSITIVE 1 homolog of pea ' Plant Journal , vol 36 , no. 3 , pp. 291-300 . , 10.1046/j.1365-313X.2003.01863.x en
dc.identifier.issn 0960-7412
dc.identifier.other PURE: 138307
dc.identifier.other dspace: 2160/3968
dc.identifier.uri http://hdl.handle.net/2160/3968
dc.description Nomura, T., Bishop, G. J., Kaneta, T., Reid, J. B., Chory, J., Yokota, T. (2003). The LKA gene is a BRASSINOSTEROID INSENSITIVE 1 homolog of pea.  Plant Journal, 36, (3), 291-300. Sponsorship: Japan Society for the Promotion of Science (grant no. 1146007 )/Human Frontier Research Program (grant no. 2000-162) en
dc.description.abstract Brassinosteroids (BRs) are growth-promoting plant steroid hormones, and in garden pea (Pisum sativum L.), the lka mutant is defective in BR perception. Here, we show that LKA encodes P. sativum BRI1 (PsBRI1), a homolog of BRI1, which is the Arabidopsis leucine-rich repeat receptor-like kinase/BR receptor. PsBRI1 was isolated by screening a pea cDNA library using Arabidopsis BRI1 cDNA as the probe. PsBRI1 is predicted to encode a 1188-amino-acid protein that has 78% similarity with Arabidopsis BRI1. Sequence analysis of PsBRI1 in the lka mutant led to the identification of a missense mutation that converts the highly conserved aspartic acid residue to asparagine, which is located in the leucine-rich repeat, just before the island domain that may bind BR or a BR–protein complex. The mutation identified in PsBRI1 co-segregated with the semi-erectoide lka phenotype. Transcript analysis of LKA/PsBRI1 indicates that it is ubiquitously expressed in pea and that the expression was downregulated by exogenous BR. The lka mutant was then utilized in further studies to analyze the independent actions of BR and gibberellin (GA) through the characterization of BR response on GA mutants and GA response on BR mutants. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Plant Journal en
dc.title The LKA gene is a BRASSINOSTEROID INSENSITIVE 1 homolog of pea en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1046/j.1365-313X.2003.01863.x
dc.contributor.institution Aberystwyth University en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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