Show simple item record Kingston-Smith, Alison H. Bollard, Andrea L. Armstead, Ian P. Thomas, Barry John Theodorou, Michael K. 2010-01-10T12:43:13Z 2010-01-10T12:43:13Z 2003-03
dc.identifier.citation Kingston-Smith , A H , Bollard , A L , Armstead , I P , Thomas , B J & Theodorou , M K 2003 , ' Proteolysis and cell death in clover leaves is induced by grazing ' Protoplasma , vol 220 , no. 3-4 , pp. 119-129 . DOI: 10.1007/s00709-002-0044-5 en
dc.identifier.issn 0033-183X
dc.identifier.other PURE: 138283
dc.identifier.other PURE UUID: 382be295-e7c6-41a2-a80b-16f68bd3133a
dc.identifier.other dspace: 2160/3969
dc.identifier.other DSpace_20121128.csv: row: 3153
dc.identifier.other Scopus: 0037345614
dc.identifier.other PubMed: 12664276
dc.description Kingston-Smith, A. H., Bollard, A. L., Armstead, I. P., Thomas, B. J., Theodorou, M. K. (2003). Proteolysis and cell death in clover leaves is induced by grazing. Protoplasma, 220 (3-4), 119-129. en
dc.description.abstract Programmed plant cell death is a widespread phenomenon resulting in the formation of xylem vessels, dissected leaf forms, and aerenchyma. We demonstrate here that some characteristics of programmed cell death can also be observed during the cellular response to biotic and abiotic stress when plant tissue is ingested by grazing ruminants. Furthermore, the onset and progression of plant cell death processes may influence the proteolytic rate in the rumen. This is important because rapid proteolysis of plant proteins in ruminants is a major cause of the inefficient conversion of plant to animal protein resulting in the release of environmental N pollutants. Although rumen proteolysis is widely believed to be mediated by proteases from rumen microorganisms, proteolysis and cell death occurred concurrently in clover leaves incubated in vitro under rumenlike conditions (maintained anaerobically at 39 °C) but in the absence of a rumen microbial population. Under rumenlike conditions, both red and white clover cells showed progressive loss of DNA, but this was only associated with fragmentation in white clover. Cell death was indicated by increased ionic leakage and the appearance of terminal deoxynucleotidyl transferase-mediated dUTP-nick-end-labelled nuclei. Foliar protein decreased to 50% of the initial values after 3 h incubation in white clover and after 4 h in red clover, while no decrease was observed in ambient (25 °C, aerobic) incubations. In white clover, decreased foliar protein coincided with an increased number of protease isoforms. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Protoplasma en
dc.rights en
dc.subject Trifolium pratense en
dc.subject Trifolium repens en
dc.subject Proteolysis en
dc.subject Rumen en
dc.subject Cell death en
dc.title Proteolysis and cell death in clover leaves is induced by grazing en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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