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dc.contributor.author Mukamolova, Galina V.
dc.contributor.author Telkov, M. V.
dc.contributor.author Bagramyan, K.
dc.contributor.author Young, Michael
dc.contributor.author Shleeva, M. O.
dc.contributor.author Kaprelyants, Arseny S.
dc.contributor.author Kell, Douglas B.
dc.date.accessioned 2010-02-05T10:03:34Z
dc.date.available 2010-02-05T10:03:34Z
dc.date.issued 2002
dc.identifier.citation Mukamolova , G V , Telkov , M V , Bagramyan , K , Young , M , Shleeva , M O , Kaprelyants , A S & Kell , D B 2002 , ' Formation and resuscitation of 'non-culturable' cells of Rhodococcus rhodochrous and Mycobacterium tuberculosis in prolonged stationary phase ' Microbiology , pp. 1581-1591 . en
dc.identifier.issn 1465-2080
dc.identifier.other PURE: 134271
dc.identifier.other dspace: 2160/4032
dc.identifier.uri http://hdl.handle.net/2160/4032
dc.identifier.uri http://mic.sgmjournals.org/cgi/content/abstract/148/5/1581 en
dc.description Shleeva, M. O., Bagramyan, K., Telkov, M. V., Mukamolova, G. V., Young, M., Kell, D. B., Kaprelyants, A. S. (2002). Formation and resuscitation of 'non-culturable' cells of Rhodococcus rhodochrous and Mycobacterium tuberculosis in prolonged stationary phase. Microbiology, 148, (5), 1581-1591 Sponsorship: Wellcome Trust (grant no. 055840): BBSRC; The Russian Foundation for Basic Research (grants 00-04-48691 and 01-04-06180mas) en
dc.description.abstract After growth of Rhodococcus rhodochrous in Sauton’s medium, and further incubation for about 60 h in stationary phase, there was a transient (up to 5 log) decrease in the c.f.u. count, whereas the total count remained similar to its initial value. At the point of minimal viability, the most probable number (MPN) count was 10 times greater than the c.f.u. count. This difference was further magnified by 3–4 logs (giving values close to the total count) by incorporating supernatant taken from growing cultures. A small protein similar to Rpf (resuscitation-promoting factor of Micrococcus luteus) appeared to be responsible for some of the activity in the culture supernatant. The formation of ‘non-culturable’ cells of the ‘Academia’ strain of Mycobacterium tuberculosis was similarly observed following growth in Sauton’s medium containing Tween 80 in sealed culture vessels, and further incubation for an extended stationary phase. This resulted in the formation, 4–5 months post-inoculation, of a homogeneous population of ostensibly ‘non-culturable’ cells (zero c.f.u.). Remarkably, the MPN count for these cultures was 105 organisms ml-1, and this value was further increased by one log using supernatant from an actively growing culture. Populations of ‘non-culturable’ cells of Mycobacterium tuberculosis were also obtained by the filtration of ‘clumpy’ cultures, which were grown in the absence of Tween 80. These small cells could only be grown in liquid medium (MPN) and their viability was enhanced by the addition of culture supernatant or Rpf. The ‘non-culturable’ cells that accumulated during prolonged stationary phase in both the R. rhodochrous and the Mycobacterium tuberculosis cultures were small ovoid and coccoid forms with an intact permeability barrier, but with undetectable respiratory activity. The authors consider these non-culturable bacteria to be dormant. The observed activity of culture supernatants and Rpf with ‘non-culturable’ bacterial suspensions invites the speculation that one, or more, of the cognate Mycobacterium tuberculosis Rpf-like molecule(s) could be involved in mechanisms of latency and reactivation of tuberculosis in vivo. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Microbiology en
dc.title Formation and resuscitation of 'non-culturable' cells of Rhodococcus rhodochrous and Mycobacterium tuberculosis in prolonged stationary phase en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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