Show simple item record Bancoş, Simona Nomura, Takahito Sato, Tatsuro Molnár, Gergely Bishop, Gerard J Koncz, Csaba Yokota, Takao Nagy, Ferenc Szekeres, Miklós 2010-02-05T13:50:50Z 2010-02-05T13:50:50Z 2002-09
dc.identifier.citation Bancoş , S , Nomura , T , Sato , T , Molnár , G , Bishop , G J , Koncz , C , Yokota , T , Nagy , F & Szekeres , M 2002 , ' Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis ' Plant Physiology , vol 130 , no. 1 , pp. 504-513 . DOI: 10.1104/pp.005439 en
dc.identifier.issn 1532-2548
dc.identifier.other PURE: 134460
dc.identifier.other PURE UUID: 74eda430-a7a1-4bc1-8ac8-38ce06fd3e90
dc.identifier.other dspace: 2160/4036
dc.identifier.other DSpace_20121128.csv: row: 2976
dc.identifier.other PubMed: 12226529
dc.identifier.other Scopus: 0036741980
dc.identifier.other PubMedCentral: PMC166582
dc.description.abstract Cytochrome P450 enzymes of the closely related CYP90 and CYP85 families catalyze essential oxidative reactions in the biosynthesis of brassinosteroid (BR) hormones. Arabidopsis CYP90B1/DWF4 and CYP90A1/CPD are responsible for respective C-22 and C-23 hydroxylation of the steroid side chain and CYP85A1 catalyzes C-6 oxidation of 6-deoxo intermediates, whereas the functions of CYP90C1/ROT3, CYP90D1, and CYP85A2 are still unknown. Semiquantitative reverse transcriptase-polymerase chain reaction analyses show that transcript levels of CYP85 and CYP90 genes are down-regulated by brassinolide, the end product of the BR biosynthesis pathway. Feedback control of the CYP90C1, CYP90D1, and CYP85A2 genes by brassinolide suggests that the corresponding enzymes might also participate in BR synthesis. CYP85 and CYP90 mRNAs show strong and transient accumulation during the 1st week of seedling development, as well as characteristic organ-specific distribution. Transcripts of CYP90A1 and CYP85A2 are preferentially represented in shoots and CYP90C1, CYP90D1, and CYP85A1 mRNAs are more abundant in roots, whereas CYP90B1 is ubiquitously expressed. Remarkably, the spatial pattern of CYP90A1 expression is maintained in the BR-insensitive cbb2 mutant, indicating the independence of organ-specific and BR-dependent regulation. Quantitative gas chromatography-mass spectrometry analysis of endogenous BRs in shoots and roots of Arabidopsis, pea (Pisum sativum), and tomato (Lycopersicon esculentum) reveal similar partitioning patterns of BR intermediates in these species. Inverse correlation between CYP90A1/CPD transcript levels and the amounts of the CYP90A1 substrate 6-deoxocathasterone in shoots and roots suggests that transcriptional regulation plays an important role in controlling BR biosynthesis. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Plant Physiology en
dc.rights en
dc.title Regulation of transcript levels of the Arabidopsis cytochrome P450 genes involved in brassinosteroid biosynthesis en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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