Dual requirement of the LjSym4 gene for mycorrhizal development in epidermal and cortical cells of Lotus japonicus roots

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dc.contributor.author Faccio, Antonella
dc.contributor.author Webb, K. Judith
dc.contributor.author Novero, Mara
dc.contributor.author Parniske, Martin
dc.contributor.author Stougaard, Jens
dc.contributor.author Genre, Andrea
dc.contributor.author Bonfante, Paola
dc.contributor.author Mulder, Lonneke
dc.date.accessioned 2010-02-22T12:51:53Z
dc.date.available 2010-02-22T12:51:53Z
dc.date.issued 2002
dc.identifier.citation Faccio , A , Webb , K J , Novero , M , Parniske , M , Stougaard , J , Genre , A , Bonfante , P & Mulder , L 2002 , ' Dual requirement of the LjSym4 gene for mycorrhizal development in epidermal and cortical cells of Lotus japonicus roots ' New Phytologist , vol 154 , no. 3 , pp. 741-749 . en
dc.identifier.issn 0028-646X
dc.identifier.other PURE: 135109
dc.identifier.other dspace: 2160/4059
dc.identifier.uri http://hdl.handle.net/2160/4059
dc.identifier.uri http://www.blackwell-synergy.com/links/doi/10.1046/j.1469-8137.2002.00424.x en
dc.description Novero, M., Faccio, A., Genre, A., Stougaard, J., Webb, K. J., Mulder, L., Parniske, M., Bonfante, P. (2002). Dual requirement of the LjSym4 gene for mycorrhizal development in epidermal and cortical cells of Lotus japonicus roots.   New Phytologist, 154, (3), 741-749. Sponsorship: Universita diTorino; Consiglio Nazionale delle Ricerche en
dc.description.abstract The LjSym4 mutation leads to Lotus japonicus plants that are defective in arbuscular mycorrhiza (AM) development. • Two alleles of LjSym4 with different phenotypic strength are compared here. The development of AM was assessed by considering five parameters related to fungal structures present in root segments from wild-type and mutant plants. The distribution of intercellular hyphae was determined using semithin sections from resin-embedded roots. Cellular interactions were investigated ultrastructurally, whereas cell wall components from the host plant were identified using immunogold labeling. • In roots of Ljsym4-1 mutant, fungal hyphae were mostly restricted to the intercellular spaces of the cortex, indicating a block to infection by mutant cortical cells, which resulted in a very low number of arbuscules. • This observation suggests the presence of an additional, genetically defined 'checkpoint' for mycorrhizal development, located at the wall of cortical cells. The LjSym4 gene is therefore required for infection of both epidermal and cortical cells by AM fungi. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof New Phytologist en
dc.subject colonization process en
dc.subject mycorrhizal quantification en
dc.subject fungal viability en
dc.subject cortical block en
dc.title Dual requirement of the LjSym4 gene for mycorrhizal development in epidermal and cortical cells of Lotus japonicus roots en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1046/j.1469-8137.2002.00424.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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