Functional genomics via metabolic footprinting: monitoring metabolite secretion by Escherichia coli tryptophan metabolism mutants using FT-IR and direct injection electrospray mass spectrometry

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dc.contributor.author Ellis, David I.
dc.contributor.author Goodacre, Royston
dc.contributor.author Kaderbhai, Naheed N.
dc.contributor.author Broadhurst, David I.
dc.contributor.author Kell, Douglas B.
dc.date.accessioned 2010-02-22T12:58:03Z
dc.date.available 2010-02-22T12:58:03Z
dc.date.issued 2003-07-18
dc.identifier.citation Ellis , D I , Goodacre , R , Kaderbhai , N N , Broadhurst , D I & Kell , D B 2003 , ' Functional genomics via metabolic footprinting: monitoring metabolite secretion by Escherichia coli tryptophan metabolism mutants using FT-IR and direct injection electrospray mass spectrometry ' Comparative and Functional Genomics , vol 4 , no. 4 , pp. 376-391 . en
dc.identifier.issn 1532-6268
dc.identifier.other PURE: 136977
dc.identifier.other dspace: 2160/4061
dc.identifier.uri http://hdl.handle.net/2160/4061
dc.description Kaderbhai, N. N., Broadhurst, D. I., Ellis, D. I., Goodacre, R., Kell, D. B. (2003). Functional genomics via metabolic footprinting: monitoring metabolite secretion by Escherichia coli tryptophan metabolism mutants using FT-IR and direct injection electrospray mass spectrometry. Comparative and Functional Genomics, 4, (4), 376-391. Sponsorship: BBSRC en
dc.description.abstract We sought to test the hypothesis that mutant bacterial strains could be discriminated from each other on the basis of the metabolites they secrete into the medium (their ‘metabolic footprint’), using two methods of ‘global’ metabolite analysis (FT–IR and direct injection electrospray mass spectrometry). The biological system used was based on a published study of Escherichia coli tryptophan mutants that had been analysed and discriminated by Yanofsky and colleagues using transcriptome analysis. Wild-type strains supplemented with tryptophan or analogues could be discriminated from controls using FT–IR of 24 h broths, as could each of the mutant strains in both minimal and supplemented media. Direct injection electrospray mass spectrometry with unit mass resolution could also be used to discriminate the strains from each other, and had the advantage that the discrimination required the use of just two or three masses in each case. These were determined via a genetic algorithm. Both methods are rapid, reagentless, reproducible and cheap, and might beneficially be extended to the analysis of gene knockout libraries. en
dc.format.extent 16 en
dc.language.iso eng
dc.relation.ispartof Comparative and Functional Genomics en
dc.title Functional genomics via metabolic footprinting: monitoring metabolite secretion by Escherichia coli tryptophan metabolism mutants using FT-IR and direct injection electrospray mass spectrometry en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1002/cfg.302
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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