Restriction Fragment Length Polymorphism Analysis of Ribosomal DNA Intergenic Regions Is Useful for Differentiating Strains of Trichophyton mentagrophytes

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dc.contributor.author Barton, Richard C.
dc.contributor.author Kelly, Steven L.
dc.contributor.author Moore, Mary K.
dc.contributor.author Evans, E. Glyn
dc.contributor.author Jackson, Colin J.
dc.contributor.author Ishizaki, Hiroshi
dc.contributor.author Mochizuki, Takashi
dc.date.accessioned 2010-03-01T16:13:56Z
dc.date.available 2010-03-01T16:13:56Z
dc.date.issued 2003-10
dc.identifier.citation Barton , R C , Kelly , S L , Moore , M K , Evans , E G , Jackson , C J , Ishizaki , H & Mochizuki , T 2003 , ' Restriction Fragment Length Polymorphism Analysis of Ribosomal DNA Intergenic Regions Is Useful for Differentiating Strains of Trichophyton mentagrophytes ' Journal of Clinical Microbiology , vol 41 , no. 10 , pp. 4583-4588 . , 10.1128/JCM.41.10.4583-4588.2003 en
dc.identifier.issn 0095-1137
dc.identifier.other PURE: 135780
dc.identifier.other dspace: 2160/4129
dc.identifier.uri http://hdl.handle.net/2160/4129
dc.description Mochizuki, T., Ishizaki, H., Barton, R. C., Moore, M. K., Jackson, C. J., Kelly, S. L., Evans, E. G. (2003). Restriction Fragment Length Polymorphism Analysis of Ribosomal DNA Intergenic Regions Is Useful for Differentiating Strains of Trichophyton mentagrophytes   Journal of Clinical Microbiology, 41, (10), 4583-4588. Sponsorship: Japanese Ministry of Education, Science and Culture en
dc.description.abstract Twenty isolates of Tricophyton mentagrophytes var. mentagrophytes and 47 isolates of T. mentagrophytes var. interdigitale, identified by morphological characteristics, were screened by restriction fragment length polymorphism (RFLP) analysis of the PCR-amplified internal transcribed spacer (ITS) region of ribosomal DNA (rDNA). Sixty isolates (14 of 20 T. mentagrophytes var. mentagrophytes isolates and 46 of 47 T. mentagrophytes var. interdigitale isolates) shared an identical ITS RFLP profile and were further investigated by using a probe targeted to the rDNA nontranscribed spacer (NTS) region. Polymorphisms were observed in the NTS regions of both T. mentagrophytes var. mentagrophytes and T. mentagrophytes var. interdigitale isolates. Twenty-three individual RFLP patterns (DNA types P-1 to P-12 and A-1 to A-11) were recognized and divided into two groups depending on the presence (P) or absence (A) of a 2.5-kb band, which correlated to a large extent with the morphological variety. Eleven of 14 T. metagrophytes var. mentagrophytes isolates were A types, and all of the 46 T. mentagrophytes var. interdigitale isolates were P types. A majority of strains (23 of 60 [38.3%]) were characterized by one RFLP pattern (pattern P-1), and eight types (P-1 to P-6, P-8, and P-9) accounted for 75% (45 of 60) of all strains, including all of the T. mentagrophytes var. interdigitale isolates. The remaining 15 types were represented by one only isolate and included all of the T. mentagrophytes var. mentagrophytes isolates. We conclude that RFLP analysis of the rDNA NTS region is a valuable technique for differentiation of T. mentagrophytes strains. Furthermore, by use of this method, there appears to be a greater degree of diversity among T. mentagrophytes var. mentagrophytes isolates than among T. mentagrophytes var. interdigitale isolates. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Journal of Clinical Microbiology en
dc.title Restriction Fragment Length Polymorphism Analysis of Ribosomal DNA Intergenic Regions Is Useful for Differentiating Strains of Trichophyton mentagrophytes en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1128/JCM.41.10.4583-4588.2003
dc.contributor.institution Information Services en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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