Abstract:
Optimizing experimental conditions for the effective analysis of intact proteins by mass spectrometry is challenging, as many analytical factors influence the spectral quality, often in very different ways for different proteins and especially with complex protein mixtures. We show that genetic search methods are highly effective in this kind of optimization and that it was possible in 6 generations with a total of
Description:
Vaidyanathan, S., Broadhurst, D. I., Kell, D. B., Goodacre, R. (2003). Explanatory optimization of protein mass spectrometry via genetic search. Analytical Chemistry, 75, (23), 6679-6686. Sponsorship: BBSRC/EPSRC