Use of earthworm casts to validate FT-IR spectroscopy as a 'sentinel' technology for high-throughput monitoring of global changes in microbial ecology

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dc.contributor.author al, et
dc.contributor.author Bailey, Mark J.
dc.contributor.author Winson, Michael K.
dc.contributor.author Huang, Wei E.
dc.contributor.author Griffith, Gareth W.
dc.contributor.author Elliott, Geoffrey N.
dc.contributor.author Worgan, Hilary
dc.contributor.author Gwynn-Jones, Dylan
dc.contributor.author Scullion, John
dc.contributor.author Goodacre, Royston
dc.contributor.author Darby, Robert M.
dc.date.accessioned 2010-03-04T15:42:06Z
dc.date.available 2010-03-04T15:42:06Z
dc.date.issued 2010-03-04
dc.identifier.citation al , Bailey , M J , Winson , M K , Huang , W E , Griffith , G W , Elliott , G N , Worgan , H , Gwynn-Jones , D , Scullion , J , Goodacre , R & Darby , R M 2010 , ' Use of earthworm casts to validate FT-IR spectroscopy as a 'sentinel' technology for high-throughput monitoring of global changes in microbial ecology ' pp. 5-6 . en
dc.identifier.other PURE: 146526
dc.identifier.other dspace: 2160/4169
dc.identifier.uri http://hdl.handle.net/2160/4169
dc.description Scullion, J., Elliott, G.N., Huang, W.E., Goodacre, R., Worgan, H., Darby, R., Bailey, M.J., Gwynn-Jones, D., Griffith, G.W., Winson, M.K., Williams, P.A., Clegg,C., Draper, J. (2003). Use of earthworm casts to validate FT-IR spectroscopy as a 'sentinel' technology for high-throughput monitoring of global changes in microbial ecology.  Pedobiologia, 47, (5-6), 440-446. The 7th International Symposium on Earthworm Ecology, Cardiff, Wales, 2002. en
dc.description.abstract This study aimed to evaluate metabolic fingerprinting by Fourier transform infrared (FT-IR) spectroscopy as a technique for investigating microbial communities and their activities in soil. FT-IR spectra from earthworm casts, and other ¿biosamples¿, were compared using multivariate cluster analyses. The work formed part of a wider study to quantify the risk of horizontal gene flow and to assess ecological impacts associated with the release of GM crops or recombinant micro-organisms. A range of samples, including pure cultures of similar soil bacteria, plant materials and earthworm casts of various ages and feeding regimes were analysed. A subset of the cast FT-IR data was compared with DGGE analysis of extracted DNA/RNA. Cluster analysis of FT-IR spectra was capable of differentiating between different bacterial, litter and cast samples. There was congruence between FT-IR and DGGE clustering for food type but not for cast age. Further detailed work on the microbial populations will be needed to investigate relationships between microbial and spectroscopy data. en
dc.format.extent 2 en
dc.language.iso eng
dc.relation.ispartof en
dc.title Use of earthworm casts to validate FT-IR spectroscopy as a 'sentinel' technology for high-throughput monitoring of global changes in microbial ecology en
dc.type Text en
dc.type.publicationtype Conference paper en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Non peer reviewed en


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