Show simple item record M. S. en_US Andy J. E. en_US Emma J. en_US B. en_US Sue J. en_US Phillip en_US 2010-03-08T14:26:57Z 2010-03-08T14:26:57Z 2003-01 en_US
dc.identifier en_US
dc.identifier.citation Dhanoa , M S , Bettany , A J E , Timms , E J , Manderyck , B , Dalton , S J & Morris , P 2003 , ' Agrobacterium tumefaciens-mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.) ' Plant Cell Reports , vol 21 , no. 5 , pp. 437-444 . , 10.1007/s00299-002-0531-3 en_US
dc.identifier.other PURE: 138174 en_US
dc.identifier.other dspace: 2160/4186 en_US
dc.description.abstract Agrobacterium tumefaciens strain LBA4404 carrying plasmid pTOK233 encoding the hygromycin resistance (hph) and #-glucuronidase (uidA) genes has been used to transform two agronomic grass species: tall fescue (Festuca arundinacea) and Italian ryegrass (Lolium multiflorum). Embryogenic cell suspension colonies or young embryogenic calli were co-cultured with Agrobacterium in the presence of acetosyringone. Colonies were grown under hygromycin selection with cefotaxime and surviving colonies plated on embryogenesis media. Eight Lolium (six independent lines) and two Festuca plants (independent lines) were regenerated and established in soil. All plants were hygromycin-resistant, but histochemical determination of GUS activity showed that only one Festuca plant and one Lolium plant expressed GUS. Three GUS-negative transgenic L. multiflorum and the two F. arundinacea plants were vernalised and allowed to flower. All three Lolium plants were male- and female-fertile, but the Festuca plants failed to produce seed. Progeny analysis of L. multiflorum showed a 24-68% inheritance of the hph and uidA genes in the three lines with no significant difference between paternal and maternal gene transmission. However, significant differences were noted between the paternal and maternal expression of hygromycin resistance. en_US
dc.format.extent 8 en_US
dc.relation.ispartof Plant Cell Reports en_US
dc.title Agrobacterium tumefaciens-mediated transformation of Festuca arundinacea (Schreb.) and Lolium multiflorum (Lam.) en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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