Show simple item record

dc.contributor.author Jackson, Colin J.
dc.contributor.author Parker, Josie E.
dc.contributor.author Lamb, David C.
dc.contributor.author Marczylo, Timothy H.
dc.contributor.author Kelly, Diane E.
dc.contributor.author Manning, Nigel L.
dc.contributor.author Kelly, Steven L.
dc.date.accessioned 2010-03-09T14:52:16Z
dc.date.available 2010-03-09T14:52:16Z
dc.date.issued 2003-02-07
dc.identifier.citation Jackson , C J , Parker , J E , Lamb , D C , Marczylo , T H , Kelly , D E , Manning , N L & Kelly , S L 2003 , ' Conservation and cloning of CYP51: a sterol 14 alpha-demethylase from Mycobacterium smegmatis ' Biochemical and Biophysical Research Communications , vol 301 , no. 2 , pp. 558-563 . , 10.1016/S0006-291X(02)03078-4 en
dc.identifier.issn 1090-2104
dc.identifier.other PURE: 137849
dc.identifier.other dspace: 2160/4205
dc.identifier.uri http://hdl.handle.net/2160/4205
dc.description Jackson, C. J., Lamb, D. C., Marczylo, T. H., Parker, J. E., Manning, N. L., Kelly, D. E., Kelly, S. L. (2003). Conservation and cloning of CYP51: a sterol 14 alpha-demethylase from Mycobacterium smegmatis. Biochemical and Biophysical Research Communications, 30301, (2), 558-563. Sponsorship: BBSRC en
dc.description.abstract The genetic locus encoding cytochrome P450 51 (CYP51; P45014DM) in Mycobacterium smegmatis is described here together with confirmation of activity in lanosterol 14α-demethylation. The protein bound azole antifungals with high affinity and the rank order based on affinity matched the ranked order for microbiological sensitivity of the organism, thus supporting a possible role for CYP51 as a target in the antimycobacterial activity of these compounds. Non-saponifiable lipids were extracted from the bacteria grown on minimal medium. Unlike a previous report using growth on complex medium, no cholesterol was detected in two strains of M. smegmatis, but a novel lipid was detected. The genetic locus of CYP51 is discussed in relation to function; it is conserved as part of a putative operon in M. smegmatis, Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium bovis and consists of six open-reading frames including two CYPs and a ferredoxin under a putative Tet-R regulated promoter. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Biochemical and Biophysical Research Communications en
dc.title Conservation and cloning of CYP51: a sterol 14 alpha-demethylase from Mycobacterium smegmatis en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/S0006-291X(02)03078-4
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Cadair


Advanced Search

Browse

Statistics