Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs

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dc.contributor.author Schramm, G.
dc.contributor.author Hamilton, J. V.
dc.contributor.author Balog, C. I. A.
dc.contributor.author Wuhrer, M.
dc.contributor.author Gronow, A.
dc.contributor.author Beckmann, S.
dc.contributor.author Wippersteg, V.
dc.contributor.author Grevelding, C. G.
dc.contributor.author Goldmann, T.
dc.contributor.author Weber, E.
dc.contributor.author Brattig, N. W.
dc.contributor.author Deelder, A. M.
dc.contributor.author Dunne, D. W.
dc.contributor.author Hokke, C. H.
dc.contributor.author Haas, H.
dc.contributor.author Doenhoff, M. J.
dc.date.accessioned 2010-03-12T15:16:33Z
dc.date.available 2010-03-12T15:16:33Z
dc.date.issued 2009-07
dc.identifier.citation Schramm , G , Hamilton , J V , Balog , C I A , Wuhrer , M , Gronow , A , Beckmann , S , Wippersteg , V , Grevelding , C G , Goldmann , T , Weber , E , Brattig , N W , Deelder , A M , Dunne , D W , Hokke , C H , Haas , H & Doenhoff , M J 2009 , ' Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs ' Molecular and Biochemical Parasitology , vol 166 , no. 1 , pp. 4-14 . , 10.1016/j.molbiopara.2009.02.003 en
dc.identifier.issn 0166-6851
dc.identifier.other PURE: 149027
dc.identifier.other dspace: 2160/4420
dc.identifier.uri http://hdl.handle.net/2160/4420
dc.description Schramm, G., Hamilton, J. V., Balog, C. I. A., Wuhrer, M., Gronow, A., Wippersteg, V., Beckmann, S., Grevelding, C .G., Goldmann, T., Weber, E., Brattig, N. W., Deelder, A. M., Dunne, D. W., Hokke, C.H., Haas, H., Doenhoff, M. J. (2009). Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs. Molecular and Biochemical Parasitology, 166 (1), 4-14. IMPF: 02.94 Sponsorship: Forschungsgemeinschaft (DFG), SFB/TR22-TP A12 en
dc.description.abstract The major immunopathological consequences of infection with Schistosoma mansoni, a T helper type 2 response and granuloma formation leading to fibrotic tissue damage, are caused by the egg stage of the parasite. Three antigens of S. mansoni eggs, termed IPSE/alpha-1, omega-1 and kappa-5, have been found to be the primary targets of the egg-directed antibody response of the host. Here, we report on the isolation, cloning and characterisation of kappa-5. Apart from an uncharacterised mRNA sequence in S. japonicum, no significant similarities of kappa-5 to known sequences from other species were found. In contrast to IPSE/alpha-1 and omega-1, which have been found only in eggs, kappa-5 was present in miracidia as well as in eggs at the mRNA and protein levels. In eggs, isoforms of kappa-5 were observed with both three and four fully occupied N-glycosylation sites, while in miracidia only one isoform with four N-glycans could be detected. Interestingly, in Western blots sera from S. mansoni-infected Africans were reactive against kappa-5 with IgE and IgG isotype antibodies, but against IPSE/alpha-1 and omega-1 only with IgG antibodies. The further characterisation of kappa-5 as one of the three major egg antigens should help to better understand the immunology and immunopathology of schistosomiasis. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Molecular and Biochemical Parasitology en
dc.subject Schistosoma mansoni en
dc.subject Kappa-5 en
dc.subject Egg antigen en
dc.subject SmEA en
dc.subject Excretory/secretory en
dc.title Molecular characterisation of kappa-5, a major antigenic glycoprotein from Schistosoma mansoni eggs en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.molbiopara.2009.02.003
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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