Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping

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dc.contributor.author Lee, David
dc.contributor.author La Mura, Maurizio
dc.contributor.author Allnutt, Theo
dc.contributor.author Powell, Wayne
dc.contributor.author Greenland, Andy
dc.date.accessioned 2010-03-12T16:37:05Z
dc.date.available 2010-03-12T16:37:05Z
dc.date.issued 2009-09-22
dc.identifier.citation Lee , D , La Mura , M , Allnutt , T , Powell , W & Greenland , A 2009 , ' Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping ' Journal of Agricultural and Food Chemistry , vol 57 , no. 20 , pp. 9400-9402 . en
dc.identifier.issn 1520-5118
dc.identifier.other PURE: 144674
dc.identifier.other dspace: 2160/4426
dc.identifier.uri http://hdl.handle.net/2160/4426
dc.identifier.uri http://pubs.acs.org/doi/abs/10.1021%2Fjf902455j en
dc.description Lee, D., La Mura, M., Allnutt, T., Powell, W., Greenland, A. (2009). Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping. Journal of Agricultural and Food Chemistry, 57 (20), 9400-9402. IMPF: 02.47 Sponsorship: EU en
dc.description.abstract DNA extractions are a major cost for high-throughput genotyping. The loop-mediated isothermal amplification (LAMP) assay has been used for the detection of two genetically modified (GM) related sequences. The amplification of target DNA sequences from leaf and maize seed tissues prepared with minimum preparative treatment (disruption in water) demonstrates the ability of LAMP to work in conditions normally inhibitive to PCRs. The wide dynamic range of detection in these samples suggests that LAMP is highly sensitive even when the target is presented in such a crude form. LAMP offers a means of reducing genotyping costs as well as simplifying testing procedures. en
dc.format.extent 3 en
dc.language.iso eng
dc.relation.ispartof Journal of Agricultural and Food Chemistry en
dc.subject Loop-mediated isothermal amplification (LAMP) en
dc.subject GM detection en
dc.subject RF3 oilseed rape en
dc.subject leaf tissue en
dc.subject MON810 maize seed en
dc.title Isothermal Amplification of Genetically Modified DNA Sequences Directly from Plant Tissues Lowers the Barriers to High-Throughput and Field-Based Genotyping en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1021/jf902455j
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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