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dc.contributor.author Mur, Luis A. J.
dc.contributor.author Donnison, Iain S.
dc.contributor.author Milbourne, Dan
dc.contributor.author Guiney, Emma
dc.contributor.author Byrne, Stephen
dc.contributor.author Barth, Susanne
dc.date.accessioned 2010-03-17T09:39:17Z
dc.date.available 2010-03-17T09:39:17Z
dc.date.issued 2009-09-17
dc.identifier.citation Mur , L A J , Donnison , I S , Milbourne , D , Guiney , E , Byrne , S & Barth , S 2009 , ' Identification of genes involved in the floral transition at the shoot apical meristem of Lolium perenne L. by use of suppression subtractive hybridisation ' Plant Growth Regulation , vol 59 , no. 3 , pp. 215-225 . , 10.1007/s10725-009-9407-7 , 10.1007/s10725-009-9407-7 en
dc.identifier.issn 1573-5087
dc.identifier.other PURE: 148587
dc.identifier.other dspace: 2160/4452
dc.identifier.uri http://hdl.handle.net/2160/4452
dc.description Byrne, S. L., Guiney, E., Donnison, I. S., Mur, L. A. J., Milbourne, D., Barth, S. (2009). Identification of genes involved in the floral transition at the shoot apical meristem of Lolium perenne L. by use of suppression subtractive hybridisation. Plant Growth Regulation, 59, (3), 215-225. Electronic supplementary material. The online version of this article (doi:10.1007/s10725-009-9407-7) contains supplementary material, which is available to authorized users. IMPF: 01.53 RONO: 03134 Sponsorship: BBSRC, TEAGASC, Ireland en
dc.description.abstract Generally, heading in Lolium perenne L. (perennial ryegrass) is associated with a reduction in the feed quality of the forage and therefore extending the period of vegetative growth during the growing season will contribute to an improvement in quality. The genetic control of floral transition has been well studied in model plant species but less research has been done in economically important crop species such as perennial ryegrass. A differential gene expression study was performed between two full sibling lines of L. perenne with contrasting flowering time. Suppression subtractive hybridization was used to identify 155 transcripts differentially expressed between the two sibling lines in the shoot apical meristem after primary and during secondary induction. Transcripts with a putative role in the floral transition were further characterized, through floral induction stages, by real time RT?PCR. This revealed five genes with greater than tenfold difference in expression between the lines during floral induction. Furthermore, a putative methyl binding domain protein and bHLH transcription factor were identified, which show clear differential expression patterns through floral induction and may act as potential enhancers of flowering in L. perenne en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Plant Growth Regulation en
dc.title Identification of genes involved in the floral transition at the shoot apical meristem of Lolium perenne L. by use of suppression subtractive hybridisation en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s10725-009-9407-7
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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