Use of secondary somatic embryos promotes genetic fidelity in cryopreservation of cocoa (Theobroma cacao L.)

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dc.contributor.author Wilkinson, Michael J.
dc.contributor.author Rodriguez Lopez, Carlos Marcelino
dc.contributor.author Adu-Gyamfi, Raphael
dc.contributor.author Fang, Jong-Yi
dc.contributor.author Wetten, Andy
dc.date.accessioned 2010-03-17T09:54:27Z
dc.date.available 2010-03-17T09:54:27Z
dc.date.issued 2009-08-01
dc.identifier.citation Wilkinson , M J , Rodriguez Lopez , C M , Adu-Gyamfi , R , Fang , J-Y & Wetten , A 2009 , ' Use of secondary somatic embryos promotes genetic fidelity in cryopreservation of cocoa (Theobroma cacao L.) ' Agricultural and Food Science , vol 18 , no. 2 (Special issue) , pp. 152-159 . , 10.2137/145960609789267579 en
dc.identifier.issn 1795-1895
dc.identifier.other PURE: 148483
dc.identifier.other dspace: 2160/4455
dc.identifier.uri http://hdl.handle.net/2160/4455
dc.identifier.uri http://www.mtt.fi/afs/pdf/mtt-afs-v18n2p152.pdf en
dc.description Fang, Jong-Yi, Wetten, A., Adu-Gyamfi, R., Wilkinson, M., Rodriguez-Lopez, C. (2009). Use of secondary somatic embryos promotes genetic fidelity in cryopreservation of cocoa (Theobroma cacao L.). Agricultural and Food Science, 18, (2 (Special issue)), 152-159. Sponsorship: COCOA RESEARCH UK IMPF: 00.92 RONO: 00 en
dc.description.abstract The inability to conserve cocoa (Theobroma cacao L.) germplasm via seed storage and the vulnerability of field collections make the establishment of cryopreserved genebanks for the crop a priority. An effective encapsulation-dehydration based cryopreservation system has been developed for cocoa but because the somatic embryos used for freezing arise after a protracted period of callus culture there is concern about maintenance of genetic fidelity during the process. Microsatellite markers for seven of the 10 cocoa linkage groups were used to screen a population of 189 primary somatic embryo-derived emblings and the 43 secondary somatic embryos they gave rise to. Of the primary somatic embryos, 38.1% exhibited polymorphic microsatellite profiles while for secondary somatic embryos the frequency was 23.3%. The same microsatellite markers used to screen another population of 44 secondary somatic embryos cryopreserved through encapsulation-dehydration revealed no polymorphisms. Scanning electron microscopy showed the secondary somatic embryos were derived from cotyledonary epidermal cells rather than callus. The influence of embryo ontogeny on somaclonal variation is discussed. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Agricultural and Food Science en
dc.title Use of secondary somatic embryos promotes genetic fidelity in cryopreservation of cocoa (Theobroma cacao L.) en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.2137/145960609789267579
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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