Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi

H...............H

Show simple item record

dc.contributor.author Jackson, C. J.
dc.contributor.author Barton, Richard C.
dc.contributor.author Clark, C. Graham
dc.contributor.author Kelly, Steven L.
dc.date.accessioned 2010-03-18T09:07:20Z
dc.date.available 2010-03-18T09:07:20Z
dc.date.issued 2009
dc.identifier.citation Jackson , C J , Barton , R C , Clark , C G & Kelly , S L 2009 , ' Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi ' Medical Mycology , vol 47 , no. 6 , pp. 609-617 . , 10.1080/13693780802385445 en
dc.identifier.issn 1460-2709
dc.identifier.other PURE: 145202
dc.identifier.other dspace: 2160/4474
dc.identifier.uri http://hdl.handle.net/2160/4474
dc.description Jackson, C. J., Barton, R. C., Clark, C. G., Kelly, S. L. (2009). Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi. Medical Mycology, 47 (6), 609-617. IMPF: 02.13 en
dc.description.abstract Group I introns have the ability to catalyse their own excision (self-splice) from pre-RNA, and are found in a wide range of eukaryotic organisms. In fungal nuclear genomes, they have been identified in the small subunit (SSU) and large subunit (LSU) of the ribosomal RNA gene. Sequencing of the 3′ region of the LSU rRNA gene of the dermatophyte Trichophyton interdigitale revealed a 393 bp group I intron, Tin.2563, containing the four characteristic conserved motifs (P,Q,R and S) essential for self-splicing. The predicted secondary structure revealed nine sets of conserved paired regions (P1–P9), with most similarity to a subgroup IE intron of the entomopathogenic hyphomycete Beauveria bassiana. Tin.2563 was inserted at a site in the LSU rDNA corresponding to position 2563 of the Escherichia coli 23S rRNA. PCR and sequence analysis showed an intron to be present at an identical location in the LSU rDNA of many dermatophytes, although its distribution was erratic. In contrast, an intron was present at the same location in multiple isolates (n = 20) of the clinically important anthrophilic species Trichophyton rubrum and T. interdigitale. Conservation of intron insertion site, subgroup and P helix sequences showed intron genotyping to be unsuitable for strain identification in dermatophytes. Phylogenetic analysis of intron sequences from different dermatophyte species indicated that lateral transfer of the element was likely to be a rare event. en
dc.format.extent 9 en
dc.language.iso eng
dc.relation.ispartof Medical Mycology en
dc.title Molecular characterization of a subgroup IE intron with wide distribution in the large subunit rRNA genes of dermatophyte fungi en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1080/13693780802385445
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Cadair


Advanced Search

Browse

My Account