Show simple item record Langdon, Tim Thomas, Ann Huang, Lin Farrar, Kerrie King, Julie Armstead, Ian 2010-03-18T15:35:54Z 2010-03-18T15:35:54Z 2009-06-07
dc.identifier.citation Langdon , T , Thomas , A , Huang , L , Farrar , K , King , J & Armstead , I 2009 , ' Fragments of the key flowering gene GIGANTEA are associated with helitron-type sequences in the Pooideae grass Lolium perenne ' BMC Plant Biology , vol 9 , 70 . DOI: 10.1186/1471-2229-9-70 en
dc.identifier.issn 1471-2229
dc.identifier.other PURE: 145447
dc.identifier.other PURE UUID: 2a847571-52fd-47e9-bea4-60a22b009c34
dc.identifier.other dspace: 2160/4483
dc.identifier.other DSpace_20121128.csv: row: 3444
dc.identifier.other IBERS: 0000017398
dc.identifier.other Ibers_20121112_1204.csv: row: 623
dc.identifier.other RAD: 1536
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 768
dc.identifier.other Scopus: 67650800330
dc.identifier.uri en
dc.description IMPF: 03.77 RONO: AC03130 Sponsorship: BBSRC en
dc.description.abstract Background Helitrons are a class of transposable elements which have been identified in a number of species of plants, animals and fungi. They are unique in their proposed rolling-circle mode of replication, have a highly variable copy-number and have been implicated in the restructuring of coding sequences both by their insertion into existing genes and by their incorporation of transcriptionally competent gene fragments. Helitron discovery depends on identifying associated DNA signature sequences and comprehensive evaluation of helitron contribution to a particular genome requires detailed computational analysis of whole genome sequence. Therefore, the role which helitrons have played in modelling non-model plant genomes is largely unknown. Results Cloning of the flowering gene GIGANTEA (GI) from a BAC library of the Pooideae grass Lolium perenne (perennial ryegrass) identified the target gene and several GI pseudogene fragments spanning the first five exons. Analysis of genomic sequence 5 and 3 of one these GI fragments revealed motifs consistent with helitron-type transposon insertion, specifically a putative 5-A T-3 insertion site containing 5-TC and CTAG-3 borders with a sub-terminal 16 bp hairpin. Screening of a BAC library of the closely related grass species Festuca pratensis (meadow fescue) indicated similar helitron-associated GI fragments present in this genome, as well as non-helitron associated GI fragments derived from the same region of GI. In order to investigate the possible extent of ancestral helitron-activity in L. perenne, a methylation-filtered GeneThresher genomic library developed from this species was screened for potential helitron 3 hairpin sequences associated with a 3-CTRR motif. This identified 7 potential helitron hairpin-types present between at least 9 and 51 times within the L. perenne methylation-filtered library. Conclusion This represents evidence for a possible ancestral role for helitrons in modelling the genomes of Lolium and related species. en
dc.language.iso eng
dc.relation.ispartof BMC Plant Biology en
dc.rights en
dc.title Fragments of the key flowering gene GIGANTEA are associated with helitron-type sequences in the Pooideae grass Lolium perenne en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.description.version publishersversion en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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