Glutathione transferase (GST) as a candidate molecular based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus

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dc.contributor.author LaCourse, E. James
dc.contributor.author Hernandez-Viadel, Mariluz
dc.contributor.author Jefferies, James R.
dc.contributor.author Svendsen, Claus
dc.contributor.author Spurgeon, David J
dc.contributor.author Barrett, John
dc.contributor.author Morgan, A. John
dc.contributor.author Kille, Peter
dc.contributor.author Brophy, Peter Michael
dc.date.accessioned 2010-03-23T15:57:30Z
dc.date.available 2010-03-23T15:57:30Z
dc.date.issued 2009-04-05
dc.identifier.citation LaCourse , E J , Hernandez-Viadel , M , Jefferies , J R , Svendsen , C , Spurgeon , D J , Barrett , J , Morgan , A J , Kille , P & Brophy , P M 2009 , ' Glutathione transferase (GST) as a candidate molecular based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus ' Environmental Pollution , vol 157 , no. 8-9 , pp. 2459-2469 . , 10.1016/j.envpol.2009.03.015 en
dc.identifier.issn 1873-6424
dc.identifier.other PURE: 146290
dc.identifier.other dspace: 2160/4507
dc.identifier.uri http://hdl.handle.net/2160/4507
dc.description LaCourse, E. J., Hernandez-Viadel, M., Jefferies, J. R., Svendsen, C., Spurgeon, D. J., Barrett, J., Kille, P., Brophy, P. M. (2009). Glutathione transferase (GST) as a candidate molecular based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus. Environmental Pollution, 157, (8-9), 2459-2469. IMPF: 03.43 RONO: 00 Sponsorship: NERC UK/ University of Wales en
dc.description.abstract The earthworm Lumbricus rubellus (Hoffmeister, 1843) is a terrestrial pollution sentinel. Enzyme activity and transcription of phase II detoxification superfamily glutathione transferases (GST) is known to respond in earthworms after soil toxin exposure, suggesting GST as a candidate molecular-based pollution biomarker. This study combined sub-proteomics, bioinformatics and biochemical assay to characterise the L. rubellus GST complement as pre-requisite to initialise assessment of the applicability of GST as a biomarker. L. rubellus possesses a range of GSTs related to known classes, with evidence of tissue-specific synthesis. Two affinity-purified GSTs dominating GST protein synthesis (Sigma and Pi class) were cloned, expressed and characterised for enzyme activity with various substrates. Electrospray ionisation mass spectrometry (ESI-MS) and tandem mass spectrometry (MS/MS) following SDS-PAGE were superior in retaining subunit stability relative to two-dimensional gel electrophoresis (2-DE). This study provides greater understanding of Phase II detoxification GST superfamily status of an important environmental pollution sentinel organism. This study currently provides the most comprehensive view of the Phase II detoxification enzyme superfamily of glutathione transferases within the important environmental pollution sentinel earthworm Lumbricus rubellus. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Environmental Pollution en
dc.subject Glutathione transferase en
dc.subject Lumbricus rubellus en
dc.subject Earthworm en
dc.subject 2-DE en
dc.title Glutathione transferase (GST) as a candidate molecular based biomarker for soil toxin exposure in the earthworm Lumbricus rubellus en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.envpol.2009.03.015
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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