Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences

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dc.contributor.author Lee, David
dc.contributor.author La Mura, Maurizio
dc.contributor.author Allnutt, Theo R.
dc.contributor.author Powell, Wayne
dc.date.accessioned 2010-03-23T16:15:14Z
dc.date.available 2010-03-23T16:15:14Z
dc.date.issued 2009-02-02
dc.identifier.citation Lee , D , La Mura , M , Allnutt , T R & Powell , W 2009 , ' Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences ' BMC Biotechnology , vol 9 , no. 7 . , 10.1186/1472-6750-9-7 en
dc.identifier.issn 1472-6750
dc.identifier.other PURE: 146313
dc.identifier.other dspace: 2160/4512
dc.identifier.uri http://hdl.handle.net/2160/4512
dc.description Lee, D., La Mura, M., Allnutt, T. R., Powell, W. (2009). Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences . BMC Biotechnology, 9, paper no. 7. IMPF: 02.72 RONO: 00 Sponsorship: EU / University of Naples en
dc.description.abstract The most common method of GMO detection is based upon the amplification of GMO-specific DNA amplicons using the polymerase chain reaction (PCR). Here we have applied the loop-mediated isothermal amplification (LAMP) method to amplify GMO-related DNA sequences, 'internal' commonly-used motifs for controlling transgene expression and event-specific (plant-transgene) junctions. We have tested the specificity and sensitivity of the technique for use in GMO studies. Results show that detection of 0.01% GMO in equivalent background DNA was possible and dilutions of template suggest that detection from single copies of the template may be possible using LAMP. This work shows that GMO detection can be carried out using LAMP for routine screening as well as for specific events detection. Moreover, the sensitivity and ability to amplify targets, even with a high background of DNA, here demonstrated, highlights the advantages of this isothermal amplification when applied for GMO detection. en
dc.language.iso eng
dc.relation.ispartof BMC Biotechnology en
dc.title Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1186/1472-6750-9-7
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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