Show simple item record David en_US Maurizio en_US Theo R. en_US Wayne en_US 2010-03-23T16:15:14Z 2010-03-23T16:15:14Z 2009-02-02 en_US
dc.identifier en_US
dc.identifier.citation Lee , D , La Mura , M , Allnutt , T R & Powell , W 2009 , ' Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences ' BMC Biotechnology , vol 9 , no. 7 . , 10.1186/1472-6750-9-7 en_US
dc.identifier.other PURE: 146313 en_US
dc.identifier.other dspace: 2160/4512 en_US
dc.description.abstract The most common method of GMO detection is based upon the amplification of GMO-specific DNA amplicons using the polymerase chain reaction (PCR). Here we have applied the loop-mediated isothermal amplification (LAMP) method to amplify GMO-related DNA sequences, 'internal' commonly-used motifs for controlling transgene expression and event-specific (plant-transgene) junctions. We have tested the specificity and sensitivity of the technique for use in GMO studies. Results show that detection of 0.01% GMO in equivalent background DNA was possible and dilutions of template suggest that detection from single copies of the template may be possible using LAMP. This work shows that GMO detection can be carried out using LAMP for routine screening as well as for specific events detection. Moreover, the sensitivity and ability to amplify targets, even with a high background of DNA, here demonstrated, highlights the advantages of this isothermal amplification when applied for GMO detection. en_US
dc.relation.ispartof BMC Biotechnology en_US
dc.title Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

Files in this item

Aside from theses and in the absence of a specific licence document on an item page, all works in Cadair are accessible under the CC BY-NC-ND Licence. AU theses and dissertations held on Cadair are made available for the purposes of private study and non-commercial research and brief extracts may be reproduced under fair dealing for the purpose of criticism or review. If you have any queries in relation to the re-use of material on Cadair, contact

This item appears in the following Collection(s)

Show simple item record

Search Cadair

Advanced Search