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dc.contributor.author David en_US
dc.contributor.author Maurizio en_US
dc.contributor.author Theo R. en_US
dc.contributor.author Wayne en_US
dc.date.accessioned 2010-03-23T16:15:14Z
dc.date.available 2010-03-23T16:15:14Z
dc.date.issued 2009-02-02 en_US
dc.identifier http://dx.doi.org/10.1186/1472-6750-9-7 en_US
dc.identifier.citation Lee , D , La Mura , M , Allnutt , T R & Powell , W 2009 , ' Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences ' BMC Biotechnology , vol 9 , no. 7 . , 10.1186/1472-6750-9-7 en_US
dc.identifier.other PURE: 146313 en_US
dc.identifier.other dspace: 2160/4512 en_US
dc.identifier.uri http://hdl.handle.net/2160/4512
dc.description.abstract The most common method of GMO detection is based upon the amplification of GMO-specific DNA amplicons using the polymerase chain reaction (PCR). Here we have applied the loop-mediated isothermal amplification (LAMP) method to amplify GMO-related DNA sequences, 'internal' commonly-used motifs for controlling transgene expression and event-specific (plant-transgene) junctions. We have tested the specificity and sensitivity of the technique for use in GMO studies. Results show that detection of 0.01% GMO in equivalent background DNA was possible and dilutions of template suggest that detection from single copies of the template may be possible using LAMP. This work shows that GMO detection can be carried out using LAMP for routine screening as well as for specific events detection. Moreover, the sensitivity and ability to amplify targets, even with a high background of DNA, here demonstrated, highlights the advantages of this isothermal amplification when applied for GMO detection. en_US
dc.relation.ispartof BMC Biotechnology en_US
dc.title Detection of genetically modified organisms (GMOs) using isothermal amplification of target DNA sequences en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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