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dc.contributor.author Haider, N.
dc.contributor.author Wilkinson, Michael J.
dc.contributor.author Allainguillaume, Joel
dc.date.accessioned 2010-03-23T16:19:08Z
dc.date.available 2010-03-23T16:19:08Z
dc.date.issued 2009-04-01
dc.identifier.citation Haider , N , Wilkinson , M J & Allainguillaume , J 2009 , ' Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: implications for the use of chloroplast transformation for biocontainment ' Current Genetics , vol 55 , no. 2 , pp. 139-150 . , 10.1007/s00294-009-0230-5 en
dc.identifier.issn 1432-0983
dc.identifier.other PURE: 146604
dc.identifier.other dspace: 2160/4513
dc.identifier.uri http://hdl.handle.net/2160/4513
dc.description Haider, N., Allainguillaume, J., Wilkinson, M. J. (2009). Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: implications for the use of chloroplast transformation for biocontainment. Current Genetics, 55, (2), 139-150. Sponsorship: DEFRA SE0236, BBSRC IAH1320 IMPF: 01.84 RONO: 00 en
dc.description.abstract Environmental concerns over the cultivation of Genetically Modified (GM) crops largely centre on the ecological consequences following gene flow to wild relatives. One attractive solution is to deploy biocontainment measures that prevent hybridization. Chloroplast transformation is the most advanced biocontainment method but is compromised by chloroplast capture (hybridization through the maternal lineage). To date, however, there is a paucity of information on the frequency of chloroplast capture in the wild. Oilseed rape (Brassica napus, AACC) frequently hybridises with wild Brassica rapa (AA, as paternal parent) and yields B. rapa-like introgressed individuals after only two generations. In this study we used chloroplast CAPS markers that differentiate between the two species to survey wild and weedy populations of B. rapa for the capture of B. napus chloroplasts. A total of 464 B. rapa plants belonging to 14 populations growing either in close proximity to B. napus (i.e. sympatric 1 km) were assessed for chloroplast capture using PCR (trnL-F) and CAPS (trnT-L-Xba I) markers. The screen revealed that two sympatric B. rapa populations included 53 plants that possessed the chloroplast of B. napus. In order to discount these B. rapa plants as F1 crop-wild hybrids, we used a C-genome-specific marker and found that 45 out of 53 plants lacked the C-genome and so were at least second generation introgressants. The most plausible explanation is that these individuals represent multiple cases of chloroplast capture following introgressive hybridisation through the female germ line from the crop. The abundance of such plants in sympatric sites thereby questions whether the use of chloroplast transformation would provide a sufficient biocontainment for GM oilseed rape in the United Kingdom. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof Current Genetics en
dc.title Spontaneous capture of oilseed rape (Brassica napus) chloroplasts by wild B. rapa: implications for the use of chloroplast transformation for biocontainment en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s00294-009-0230-5
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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