A DNA Barcode for land plants

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A DNA Barcode for land plants

Hollingsworth, P. M.; Forrest, L. L.; Spouge, J. L.; Hajibabaei, M.; Ratnasingham, S.; van der Bank, M.; Chase, M. W.; Cowan, R. S.; Erickson, D. L.; Fazekas, A. J.; Graham, S. W.; James, K. E.; Kim, K. -J; Kress, W. J.; Schneider, H.; van Alphen Stahl, J.; Barrett, S. C. H.; van den Berg, C.; Bogarin, D.; Burgess, K. S.; Cameron, K. M.; Carine, M.; Chacon, J.; Clark, A. C.; Conrad, F.; Devey, D.; Clarkson, J.; Ford, C. S.; Hedderson, T. A. J.; Hollingsworth, M. L.; Husband, B. C.; Kelly, L. J.; Kesanakurti, P. R.; Kim, J. S.; Kim, Y. D.; Lahaye, R.; -L, Lee H.; Long, D. G.; Madrigan, S.; Maurin, O.; Meusnier, I.; Newmaster, S. G.; Park, C. -W; Percy, D. M.; Petersen, G.; Richardson, J. E.; Salazar, G. A.; Savolainen, V.; Seberg, O.; Wilkinson, M. J.; Yi, D. -K; Little, D. P.
URI: http://hdl.handle.net/2160/4529
Date: 2009

Abstract:

DNA barcoding involves sequencing a standard region of DNA as a tool for species identification. However, there has been no agreement on which region(s) should be used for barcoding land plants. To provide a community recommendation on a standard plant barcode, we have compared the performance of 7 leading candidate plastid DNA regions (atpF–atpH spacer, matK gene, rbcL gene, rpoB gene, rpoC1 gene, psbK–psbI spacer, and trnH–psbA spacer). Based on assessments of recoverability, sequence quality, and levels of species discrimination, we recommend the 2-locus combination of rbcL+matK as the plant barcode. This core 2-locus barcode will provide a universal framework for the routine use of DNA sequence data to identify specimens and contribute toward the discovery of overlooked species of land plants.

Description:

Hollingsworth, P. M., Forrest, L. L., Spouge, J. L., Hajibabaei, M., Ratnasingham, S., van der Bank, M., Chase, M. W., Cowan, R.S., Erickson, D. L., Fazekas, A. J., Graham, S.W., James, K. E., Kim, K.-J., Kress, W. J., Schneider, H., van Alphen Stahl, J., Barrett, S. C. H., van den Berg, C., Bogarin, D., Burgess, K. S., Cameron, K. M., Carine, M., Chacon, J., Clark, A. C., Conrad, F., Devey, D., Clarkson, J., Ford, C. S., Hedderson, T. A. J., Hollingsworth, M. L., Husband, B. C., Kelly, L. J., Kesanakurti, P. R., Kim, J. S., Kim, Y. D., Lahaye, R., Lee H.-L., Long, D. G., Madrigan, S., Maurin, O., Meusnier, I., Newmaster, S. G, Park ,C.-W., Percy, D. M., Petersen, G., Richardson, J. E., Salazar, G. A., Savolainen, V., Seberg, O., Wilkinson, M. J., Yi, D.-K., Little, D. P. (2009). A DNA Barcode for land plants. Proceedings of the National Academy of Sciences of the USA, 106, (31), 12794-12797. Sponsorship: Alfred P. Sloan Foundation, Gordon and Betty Moore Foundation, Genome Canada, Scottish Government's Rural and Environment Research and Analysis Directorate, Royal Society, South African National Research Foundation, Intramural Research Program of the National Library of Medicine, National Institutes of Health, and Consortium for the Barcode of Life.

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