Show simple item record Ford, Caroline S. Ayres, Karen L. Toomey, Nicola Haider, Nadia Stahl, Jonathan Van Alphen Kelly, Laura J. Wikström, Niklas Hollingsworth, Peter M. Duff, R. Joel Hoot, Sarah B. Cowan, Robyn S. Chase, Mark W. Wilkinson, Michael J. 2010-03-24T16:58:15Z 2010-03-24T16:58:15Z 2009-01-15
dc.identifier.citation Ford , C S , Ayres , K L , Toomey , N , Haider , N , Stahl , J V A , Kelly , L J , Wikström , N , Hollingsworth , P M , Duff , R J , Hoot , S B , Cowan , R S , Chase , M W & Wilkinson , M J 2009 , ' Selection of candidate coding DNA barcoding regions for use on land plants ' Botanical Journal of the Linnean Society , vol 159 , no. 1 , pp. 1-11 . , 10.1111/j.1095-8339.2008.00938.x en
dc.identifier.issn 1095-8339
dc.identifier.other PURE: 147919
dc.identifier.other dspace: 2160/4536
dc.description Ford, C. S., Ayres, K. L., Toomey, N., Haider, N., Stahl, J. V., Kelly, L. J., Wikstrom, N., Hollingsworth, P. M., Duff, R. J., Hoot, S. B., Cowan, R. S., Chase, M. W., Wilkinson, M. J. (2009). Selection of candidate coding DNA barcoding regions for use on land plants. Botanical Journal of the Linnean Society, 159, (1), 1-11. Sponsorship: Alfred P. Sloan Foundation Gordon and Betty Moore Foundation IMPF: 00.98 RONO: 00 en
dc.description.abstract An in silico screen of 41 of the 81 coding regions of the Nicotiana plastid genome generated a shortlist of 12 candidates as DNA barcoding loci for land plants. These loci were evaluated for amplification and sequence variation against a reference set of 98 land plant taxa. The deployment of multiple primers and a modified multiplexed tandem polymerase chain reaction yielded 85–94% amplification across taxa, and mean sequence differences between sister taxa of 6.1 from 156 bases of accD to 22 from 493 bases of matK. We conclude that loci should be combined for effective diagnosis, and recommend further investigation of the following six loci: matK, rpoB, rpoC1, ndhJ, ycf5 and accD. en
dc.format.extent 11 en
dc.language.iso eng
dc.relation.ispartof Botanical Journal of the Linnean Society en
dc.title Selection of candidate coding DNA barcoding regions for use on land plants en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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