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dc.contributor.author Parker, David A
dc.contributor.author Beckmann, Manfred
dc.contributor.author Zubair, Hassan
dc.contributor.author Enot, David Pierre Louis
dc.contributor.author Caracuel-Rios, Zaira
dc.contributor.author Overy, David P.
dc.contributor.author Snowdon, Stuart
dc.contributor.author Talbot, Nicholas J.
dc.contributor.author Draper, John
dc.date.accessioned 2010-03-25T10:12:36Z
dc.date.available 2010-03-25T10:12:36Z
dc.date.issued 2009-09
dc.identifier.citation Parker , D A , Beckmann , M , Zubair , H , Enot , D P L , Caracuel-Rios , Z , Overy , D P , Snowdon , S , Talbot , N J & Draper , J 2009 , ' Metabolomic analysis reveals a common pattern of metabolic re-programming during invasion of three host plant species by Magnaporthe grisea ' Plant Journal , vol 59 , no. 5 , pp. 723-737 . , 10.1111/j.1365-313X.2009.03912.x en
dc.identifier.issn 0960-7412
dc.identifier.other PURE: 147656
dc.identifier.other dspace: 2160/4554
dc.identifier.uri http://hdl.handle.net/2160/4554
dc.description Parker, D., Beckmann, M., Zubair, H., Enot, D. P., Caracuel-Rios, Z., Overy, D. P., Snowdon, S., Talbot, N. J., Draper, J. (2009). Metabolomic analysis reveals a common pattern of metabolic re-programming during invasion of three host plant species by Magnaporthe grisea. Plant Journal, 59 (5), 723-737. IMPF: 06.95 Sponsorship: BBSRC - BB/D006953/1 and BB/D006791/1 en
dc.description.abstract The mechanisms by which biotrophic and hemi-biotrophic fungal pathogens simultaneously subdue plant defences and sequester host nutrients are poorly understood. Using metabolite fingerprinting, we show that Magnaporthe grisea, the causal agent of rice blast disease, dynamically re-programmes host metabolism during plant colonization. Identical patterns of metabolic change occurred during M. grisea infections in barley, rice and Brachypodium distachyon. Targeted metabolite profiling by GC-MS confirmed the modulation of a conserved set of metabolites. In pre-symptomatic tissues, malate and polyamines accumulated, rather than being utilized to generate defensive reactive oxygen species, and the levels of metabolites associated with amelioration of redox stress in various cellular compartments increased dramatically. The activity of NADP-malic enzyme and generation of reactive oxygen species were localized to pathogen penetration sites, and both appeared to be suppressed in compatible interactions. Early diversion of the shikimate pathway to produce quinate was observed, as well as accumulation of non-polymerized lignin precursors. These data are consistent with modulation of defensive phenylpropanoid metabolism by M. grisea and the inability of susceptible hosts to mount a hypersensitive reaction or produce lignified papillae (both involving reactive oxygen species) to restrict pathogen invasion. Rapid proliferation of M. grisea hyphae in plant tissue after 3 days was associated with accelerated nutrient acquisition and utilization by the pathogen. Conversion of photoassimilate into mannitol and glycerol for carbon sequestration and osmolyte production appear to drive hyphal growth. Taken together, our results suggest that fungal pathogens deploy a common metabolic re-programming strategy in diverse host species to suppress plant defence and colonize plant tissue. en
dc.format.extent 15 en
dc.language.iso eng
dc.relation.ispartof Plant Journal en
dc.subject metabolome re-programming en
dc.subject host susceptible response en
dc.subject Magnaporthe grisea en
dc.subject Brachypodium distachyon en
dc.subject rice en
dc.subject barley en
dc.title Metabolomic analysis reveals a common pattern of metabolic re-programming during invasion of three host plant species by Magnaporthe grisea en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1111/j.1365-313X.2009.03912.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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