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Browsing Gwyddorau Biolegol, Amgylcheddol a Gwledig / Biological, Environmental & Rural Sciences by Author

Browsing Gwyddorau Biolegol, Amgylcheddol a Gwledig / Biological, Environmental & Rural Sciences by Author "Caligari, Peter D. S."

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BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping

Wilkinson, Michael J.; Caligari, Peter D. S.; Dear, P. H.; Vu, Giang T. H. (2010-02-08)

Physical and linkage mapping underpin efforts to sequence and characterize the genomes of eukaryotic organisms by providing a skeleton framework for whole genome assembly. Hitherto, linkage and physical 'contig' maps were ...
High-resolution melt analysis to identify and map sequence-tagged site anchor points onto linkage maps: a white lupin (Lupinus albus) map as an exemplar

Caligari, Peter D. S.; Rogers, Tom; Croxford, Adam E.; Wilkinson, Michael J. (2008-08-05)

The provision of sequence-tagged site (STS) anchor points allows meaningful comparisons between mapping studies but can be a time-consuming process for nonmodel species or orphan crops. • Here, the first use of high-resolution ...
A simple, high throughput method to locate single copy sequences from Bacterial Artificial Chromosome (BAC) libraries using High Resolution Melt analysis

Caligari, Peter D. S.; Wilkinson, Michael J.; Vu, Giang T. H. (2010-05-12)

Background The high-throughput anchoring of genetic markers into contigs is required for many ongoing physical mapping projects. Multidimentional BAC pooling strategies for PCR-based screening of large insert libraries is ...

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