Browsing Gwyddorau Biolegol, Amgylcheddol a Gwledig / Biological, Environmental & Rural Sciences by Author "Hasterok, Robert"

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  • Donnison, Iain S.; King, Ian P.; Wolny, Elzbieta; Jenkins, Glyn M.; Marasek, Agnieszka; Armstead, Ian P.; Hasterok, Robert; Idziak, Dominika; Draper, John H.; Thomas, Ann M. (2006-05-01)
    As part of an initiative to develop Brachypodium distachyon as a genomic 'bridge' species between rice and the temperate cereals and grasses, a BAC library has been constructed for the two diploid (2n = 2x = 10) genotypes, ...
  • Jenkins, Glyn M.; Hasterok, Robert (2007-02-15)
    Fluorescence in situ hybridization (FISH) using bacterial artificial chromosomes (BACs) with large genomic DNA inserts as probes (BAC 'landing') is a powerful means by which eukaryotic genomes can be physically mapped and ...
  • Bablak, Pauline; Jenkins, Glyn M.; Ghosh-Biswas, Gadab C.; Routledge, Andrew P. M.; Mur, Luis A. J.; Draper, John H.; Hasterok, Robert (2001-12-06)
    A new model for grass functional genomics is described based on Brachypodium distachyon, which in the evolution of the Pooideae diverged just prior to the clade of 'core pooid' genera that contain the majority of important ...
  • Hasterok, Robert; Langdon, Tim; Taylor, Steve; Jenkins, Glyn M. (2002)
    This paper demonstrates a simple but effective use of combinatorial probes to label plant chromosomes by multicolour fluorescence in situ hybridisation (FISH). Three different DNA probes were labelled with only two different ...
  • Wolny, Elzbieta; Lesniewska, Karolina; Hasterok, Robert; Langdon, Timothy (2011-04-01)
    The temperate annual grass Brachypodium distachyon is a diploid species with a chromosome base number of 5. It is strikingly different from other Eurasian species of the genus, which are perennial and often polyploid, with ...
  • Garvin, David F.; Mur, Luis A. J.; Hasterok, Robert; Jenkins, Glyn M.; Gu, Yong-Qiang Q.; Hazen, Samuel P.; Mockler, Tod C.; Vogel, John P. (2008-03-01)
    Grass crop genomics research frequently is hindered by large genome sizes and polyploidy. While rice is an attractive system for grass genomics due to its small genome size and available genome sequence, it is not particularly ...
  • Catalán, Pilar; Müller, Jochen; Hasterok, Robert; Jenkins, Glyn; Mur, Luis A. J.; Langdon, Timothy; Betekhtin, Alexander; Siwinska, Dorota; Pimentel, Manuel; López-Alvarez, Diana (2012-01-01)
    Background and Aims Brachypodium distachyon is being widely investigated across the world as a model plant for temperate cereals. This annual plant has three cytotypes (2n = 10, 20, 30) that are still regarded as part of ...
  • Mur, Luis Alejandro Jose; Allainguillaume, Joel; Catalán, Pilar; Hasterok, Robert; Jenkins, Glyn; Lesniewska, Karolina; Thomas, Ianto; Vogel, John P. (2011-07)
    It is now a decade since Brachypodium distachyon (Brachypodium) was suggested as a model species for temperate grasses and cereals. Since then transformation protocols, large expressed sequence tag (EST) databases, tools ...
  • Langdon, Tim; Jenkins, Glyn M.; Hasterok, Robert; Jones, R. Neil; King, Ian P. (2003-03-01)
    A lineage of CACTA family transposons has been identified in temperate grasses and cereals, and a full-length representative of the subfamily from Lolium perenne has been sequenced. Both the size and internal organization ...
  • Hasterok, Robert; Draper, John H.; Jenkins, Glyn M. (2004-05-01)
    Brachypodium distachyon is a ubiquitous, temperate grass species which is being developed and exploited as an alternative model to rice, in order to gain access to important syntenic regions of the genomes of less tractable ...
  • Hasterok, Robert; Dulawa, Joanna; Jenkins, Glyn M.; Leggett, J. Michael; Langdon, Tim (2006-03-20)
    Background: A modification of a standard method of fluorescence in situ hybridisation (FISH) is described, by which a combination of several substrates and probes on single microscope slides enables more accurate comparisons ...
  • Hasterok, Robert; Jenkins, Glyn M.; Langdon, Tim; Jones, R. Neil (2002-01-01)
    Translocations of A chromosome-specific and B chromosome-specific satellite DNA were tracked by fluorescence in situ hybridisation from an irradiated M1 generation of an experimental population of rye (Secale cereale L.) ...
  • Idziak, Dominika Ewa; Betekhtin, Alexander; Wolny, Elzbieta; Lesniewska, Karolina; Wright, Jonathan; Febrer, Melanie; Bevan, Michael W.; Jenkins, Glyn; Hasterok, Robert (2011-10)
    Chromosome painting is one of the most powerful and spectacular tools of modern molecular cytogenetics, enabling complex analyses of nuclear genome structure and evolution. For many years, this technique was restricted to ...
  • Hasterok, Robert; Jenkins, Glyn M.; Langdon, Tim; Jones, R. Neil; Maluszynska, J. (2001-09)
    Simultaneous fluorescence in situ hybridisation with 5S and 25S rDNA probes enables the discrimination of a substantial number of chromosomes of the complement of all diploid and tetraploid Brassica species of the “U-triangle”, ...

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