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dc.contributor.author Williams, Debbie J.
dc.contributor.author Williams, Gary W.
dc.contributor.author Dunne, David W.
dc.contributor.author Johnston, David A.
dc.contributor.author Fitzpatrick, Jennifer M.
dc.contributor.author Hoffmann, Karl F.
dc.contributor.author Freeman, Tom C.
dc.date.accessioned 2010-10-07T16:00:36Z
dc.date.available 2010-10-07T16:00:36Z
dc.date.issued 2010-10-07
dc.identifier.citation Williams , D J , Williams , G W , Dunne , D W , Johnston , D A , Fitzpatrick , J M , Hoffmann , K F & Freeman , T C 2010 , ' An oligonucleotide microarray for transcriptome analysis of Schistosoma mansoni and its application/use to investigate gender-associated gene expression ' Molecular & Biochemical Parasitology , pp. 1-13 . en
dc.identifier.other PURE: 160984
dc.identifier.other dspace: 2160/5765
dc.identifier.uri http://hdl.handle.net/2160/5765
dc.description Fitzpatrick, J. M., Johnston, D. A., Williams, G. W., Williams, D. J., Freeman, T. C., Dunne, D. W., Hoffmann, K. F. (2005). An oligonucleotide microarray for transcriptome analysis of Schistosoma mansoni and its application/use to investigate gender-associated gene expression. Molecular & Biochemical Parasitology, 141 (1), pp. 1-13 en
dc.description.abstract Global profiling transcriptomes of parasitic helminths offers the potential to simultaneously identify co-ordinately expressed genes, novel genetic programs and uniquely utilized metabolic pathways, which together provide an extensive and new resource for vaccine and drug discovery. We have exploited this post-genomic approach to fabricate the first oligonucleotide DNA microarray for gene expression analysis of the parasitic trematode Schistosoma mansoni. A total of 17,329 S. mansoni DNA sequences were used to design a microarray consisting of 7335 parasite elements or approximately 50% of this parasite¿s transcriptome. Here, we describe the design of this new microarray resource and its evaluation by extending studies into gender-associated gene expression in adult schistosomes.We demonstrate a high degree of reproducibility in detecting transcriptional differences among biologically replicated experiments and the ability of the microarray to distinguish between the expression of closely related gene family members. Importantly, for issues related to sexual dimorphism, labour division, gamete production and drug target discovery, 197 transcripts demonstrated a gender-biased pattern of gene expression in the adult schistosome, greatly extending the number of sex-associated genes. These data demonstrate the power of this new resource to facilitate a greater understanding into the biological complexities of schistosome development and maturation useful for identifying novel intervention strategies. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof Molecular & Biochemical Parasitology en
dc.title An oligonucleotide microarray for transcriptome analysis of Schistosoma mansoni and its application/use to investigate gender-associated gene expression en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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