Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family

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dc.contributor.author Chalmers, Iain Wyllie
dc.contributor.author McArdle, Andrew J.
dc.contributor.author Coulson, Richard M. R.
dc.contributor.author Wagner, Marissa A.
dc.contributor.author Schmid, Ralph
dc.contributor.author Hirai, Hirohisa
dc.contributor.author Hoffmann, Karl Francis
dc.date.accessioned 2010-10-11T11:33:41Z
dc.date.available 2010-10-11T11:33:41Z
dc.date.issued 2008-02-23
dc.identifier.citation Chalmers , I W , McArdle , A J , Coulson , R M R , Wagner , M A , Schmid , R , Hirai , H & Hoffmann , K F 2008 , ' Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family ' BMC Genomics , vol 9 , no. 89 , 89 . , 10.1186/1471-2164-9-89 en
dc.identifier.issn 1471-2164
dc.identifier.other PURE: 151596
dc.identifier.other dspace: 2160/5771
dc.identifier.uri http://hdl.handle.net/2160/5771
dc.identifier.uri http://www.biomedcentral.com/bmcgenomics en
dc.description Chalmers, I. W., McArdle, A. J., Coulson, R. M. R., Wagner, M. A., Schmid, R., Hirai, H., & Hoffmann, K. F. (2008). Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family. BMC Genomics, 9(89), paper no: 89. Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family. BMC Genomics On file IMPF: 03.93 Sponsorship: Wellcome Trust Career Development Grant (RG35177) awarded to KFH and Biodiversity Research of the 21st Century COE (A14) and Global COE (A06) Grants awarded to HH. en
dc.description.abstract Background: The Sperm-coating protein/Tpx-1/Ag5/PR-1/Sc7 (SCP/TAPS) domain is foundacross phyla and is a major structural feature of insect allergens, mammalian sperm proteins andparasitic nematode secreted molecules. Proteins containing this domain are implicated in diversebiological activities and may be important for chronic host/parasite interactions. Results: We report the first description of an SCP/TAPS gene family (Schistosoma mansoni venomallergen-like (SmVALs)) in the medically important Platyhelminthes (class Trematoda) and describeindividual members' phylogenetic relationships, genomic organization and life cycle expressionprofiles. Twenty-eight SmVALs with complete SCP/TAPS domains were identified and comparisonof their predicted protein features and gene structures indicated the presence of two distinct subfamilies(group 1 & group 2). Phylogenetic analysis demonstrated that this group 1/group 2 split iszoologically widespread as it exists across the metazoan sub-kingdom. Chromosomal localisationand PCR analysis, coupled to inspection of the current S. mansoni genomic assembly, revealed thatmany of the SmVAL genes are spatially linked throughout the genome. Quantitative lifecycleexpression profiling demonstrated distinct SmVAL expression patterns, including transcriptsspecifically associated with lifestages involved in definitive host invasion, transcripts restricted tolifestages involved in the invasion of the intermediate host and transcripts ubiquitously expressed.Analysis of SmVAL6 transcript diversity demonstrated statistically significant, developmentallyregulated, alternative splicing. Conclusion: Our results highlight the existence of two distinct SCP/TAPS protein types withinthe Platyhelminthes and across taxa. The extensive lifecycle expression analysis indicates severalSmVAL transcripts are upregulated in infective stages of the parasite, suggesting that theseparticular protein products may be linked to the establishment of chronic host/parasiteinteractions. en
dc.language.iso eng
dc.relation.ispartof BMC Genomics en
dc.title Developmentally regulated expression, alternative splicing and distinct sub-groupings in members of the Schistosoma mansoni venom allergen-like (SmVAL) gene family en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1186/1471-2164-9-89
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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