Exploiting plant somatic hybrids for physical mappings of ESTs


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dc.contributor.author Wardrop, J.
dc.contributor.author Fuller, J.
dc.contributor.author Machray, G. C.
dc.contributor.author Powell, Wayne
dc.date.accessioned 2010-10-12T13:29:31Z
dc.date.available 2010-10-12T13:29:31Z
dc.date.issued 2003-09-25
dc.identifier.citation Wardrop , J , Fuller , J , Machray , G C & Powell , W 2003 , ' Exploiting plant somatic hybrids for physical mappings of ESTs ' Theoretical and Applied Genetics , pp. 343-348 . , 10.1007/s00122-003-1434-6 en
dc.identifier.issn 1432-2242
dc.identifier.other PURE: 140930
dc.identifier.other dspace: 2160/5794
dc.identifier.uri http://hdl.handle.net/2160/5794
dc.description Wardrop, J., Fuller, J., Powell, W., Machray, G.C. (2004). Exploiting plant somatic hybrids for physical mappings of ESTs. Theoretical and Applied Genetics, 108, (2), 343-348. en
dc.description.abstract Methods are described for the optimisation of the generation of radiation hybrids suitable for physical mapping of a plant (barley) genome. A combination of PCR-based technologies, involving the use of whole genome, mixed primer and hemi-nested primer amplifications, can greatly extend their utility for the physical mapping of expressed sequence tags (ESTs). Using panels of hybrids and ESTs, donor DNA retention and individual marker retention frequencies for the expressed portion of the barley genome in the hybrids were estimated. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Theoretical and Applied Genetics en
dc.title Exploiting plant somatic hybrids for physical mappings of ESTs en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s00122-003-1434-6
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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