A quantitative genomic imbalance gene expression assay in a hexaploid species : wheat (Triticum aestivum)

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dc.contributor.author Stamati, K.
dc.contributor.author Mackay, I.
dc.contributor.author Powell, W.
dc.date.accessioned 2010-10-27T13:46:15Z
dc.date.available 2010-10-27T13:46:15Z
dc.date.issued 2009-01-07
dc.identifier.citation Stamati , K , Mackay , I & Powell , W 2009 , ' A quantitative genomic imbalance gene expression assay in a hexaploid species : wheat (Triticum aestivum) ' Genome , vol 52 , no. 1 , pp. 89-94 . , 10.1139/G08-096 en
dc.identifier.issn 1480-3321
dc.identifier.other PURE: 148552
dc.identifier.other dspace: 2160/5845
dc.identifier.uri http://hdl.handle.net/2160/5845
dc.description Stamati, K., Mackay, I., Powell, W. (2009). A quantitative genomic imbalance gene expression assay in a hexaploid species: wheat (Triticum aestivum). Genome, National Research Council Canada, 52, (1), 89-94. IMPF: 01.71 en
dc.description.abstract Responses to allopolyploidy include unequal expression of duplicated genes, gene silencing, and sometimes genomic rearrangements. In plants, the relationship between allelic expression differences arising from changes in regulatory regions and the resulting phenotype is poorly understood because of the complexity of their genomes and lack of efficient methodology to identify regulatory variation. Identifying functionally important regulatory variation in crops such as hexaploid wheat (Triticum aestivum) is in its infancy. More knowledge is required about the contribution of participatory genomes to its transcriptome. In this paper, we demonstrate the use of allelic imbalance assays to quantify relative expression levels across tissues and growth regimes of homoeologous transcripts of the A, B, and D genomes. Polymorphisms in the type I thionins have been used as an example. We show that expression levels vary markedly and interactively over all factors. For this gene, the B genome is the smallest contributor to the transcriptome and the D genome the largest. As additional sequence information is accumulated across genomes, this assay will allow the simple study of relative expression across multiple homeologous loci. en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Genome en
dc.subject hexaploid wheat en
dc.subject genomic contribution en
dc.subject homoeologous gene expression en
dc.subject allelic imbalance assays en
dc.title A quantitative genomic imbalance gene expression assay in a hexaploid species : wheat (Triticum aestivum) en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1139/G08-096
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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