Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samples

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dc.contributor.author Favé, Gaëlle
dc.contributor.author Beckmann, Manfred
dc.contributor.author Lloyd, Amanda
dc.contributor.author Zhou, Shaobo
dc.contributor.author Harold, Graham
dc.contributor.author Lin, Wanchang
dc.contributor.author Tailliart, Kathleen
dc.contributor.author Xie, Long
dc.contributor.author Draper, John
dc.contributor.author Mathers, John C.
dc.date.accessioned 2011-05-31T09:03:34Z
dc.date.available 2011-05-31T09:03:34Z
dc.date.issued 2011-12-01
dc.identifier.citation Favé , G , Beckmann , M , Lloyd , A , Zhou , S , Harold , G , Lin , W , Tailliart , K , Xie , L , Draper , J & Mathers , J C 2011 , ' Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samples ' Metabolomics , vol 7 , no. 4 , pp. 469-484 . en
dc.identifier.issn 1573-3890
dc.identifier.other PURE: 161655
dc.identifier.other dspace: 2160/6817
dc.identifier.uri http://hdl.handle.net/2160/6817
dc.description Fave, G., Beckmann, M., Lloyd, A. J., Zhou, S., Harold, G., Lin, W., Tailliart, K., Xie, L., Draper, J., Mathers, J. C. (2011). Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samples. IMPF: 04.50 RONO: 00 en
dc.description.abstract Conventional tools for measuring dietary exposure have well recognized limitations. Measurement of food-derived metabolites in biofluids provides an alternative approach and our aim was to develop an experimental protocol which ensures that extraneous variability does not obscure metabolic signals from ingested foods. Healthy adults consumed a standardized meal in the evening before each test day and collected pooled overnight urine. On each test day of three different studies, urine was collected in the fasted state and at different time points after consumption of a standardized breakfast. Metabolite fingerprinting of samples using Flow Infusion Electrospray-Ionization Mass Spectrometry followed by multivariate data analysis showed strong discrimination between overnight, fasting and postprandial samples, in each study separately and when data from the three studies were pooled. Such differences were robust and highly reproducible within individuals on separate occasions. Urine volume was an efficient data normalization factor for metabolite fingerprinting data. Postprandial urines had a stable chemical composition over a period of 2–4 h after eating a standardized breakfast, suggesting that there is a flexible time window for urine collection. Fasting urine samples provided a stable baseline for universal comparisons with postprandial samples. A dietary exposure biomarker discovery protocol was validated by demonstrating that top-ranked signals discriminating between fasting and 2–4 h postprandial urine samples could be linked to metabolites abundant in some components of the standardized breakfast. We conclude that the protocol developed will have value in the search for biomarker leads of dietary exposure. en
dc.format.extent 16 en
dc.language.iso eng
dc.relation.ispartof Metabolomics en
dc.subject Dietary exposure en
dc.subject Metabolomics en
dc.subject Fasting metabolite profile en
dc.subject Postprandial metabolite profile en
dc.subject Test meal paradigm en
dc.subject Multivariate data analysis en
dc.title Development and validation of a standardized protocol to monitor human dietary exposure by metabolite fingerprinting of urine samples en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s11306-011-0289-0
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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