Structural changes and cellular localization of resuscitation promoting factor in environmental isolates of Micrococcus luteus

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dc.contributor.author Koltunov, Viktoria
dc.contributor.author Greenblatt, Charles L.
dc.contributor.author Klein, Benjamin Y.
dc.contributor.author Goncharenko, Anna. V.
dc.contributor.author Demina, Galya R.
dc.contributor.author Kaprelyants, Arseny S.
dc.contributor.author Young, Michael
dc.date.accessioned 2011-05-31T13:18:39Z
dc.date.available 2011-05-31T13:18:39Z
dc.date.issued 2010-02-01
dc.identifier.citation Koltunov , V , Greenblatt , C L , Klein , B Y , Goncharenko , A V , Demina , G R , Kaprelyants , A S & Young , M 2010 , ' Structural changes and cellular localization of resuscitation promoting factor in environmental isolates of Micrococcus luteus ' Microbial Ecology , vol 59 , no. 2 , pp. 296-310 . en
dc.identifier.issn 1432-184X
dc.identifier.other PURE: 164477
dc.identifier.other dspace: 2160/6837
dc.identifier.uri http://hdl.handle.net/2160/6837
dc.identifier.uri http://www.springerlink.com/content/b25111257046k152/ en
dc.description Koltunov, V., Greenblatt, C.L., Goncharenko, A.V., Demina, G.R., Klein, B.Y., Young, M., Kaprelyants, A.S. (2010). Structural changes and cellular localization of resuscitation promoting factor in environmental isolates of Micrococcus luteus. Microbial Ecology, 59, (2), 296-310. Sponsorship: Center for the Study of Emerging Diseases/ Russian Academy of Science IMPF: 02.87 RONO: 00 en
dc.description.abstract Dormancy among nonsporulating actinobacteria is now a widely accepted phenomenon. In Micrococcus luteus, the resuscitation of dormant cells is caused by a small secreted protein (resuscitation-promoting factor, or Rpf) that is found in “spent culture medium.” Rpf is encoded by a single essential gene in M. luteus. Homologs of Rpf are widespread among the high G + C Gram-positive bacteria, including mycobacteria and streptomycetes, and most organisms make several functionally redundant proteins. M. luteus Rpf comprises a lysozyme-like domain that is necessary and sufficient for activity connected through a short linker region to a LysM motif, which is present in a number of cell-wall-associated enzymes. Muralytic activity is responsible for resuscitation. In this report, we characterized a number of environmental isolates of M. luteus, including several recovered from amber. There was substantial variation in the predicted rpf gene product. While the lysozyme-like and LysM domains showed little variation, the linker region was elongated from ten amino acid residues in the laboratory strains to as many as 120 residues in one isolate. The genes encoding these Rpf proteins have been characterized, and a possible role for the Rpf linker in environmental adaptation is proposed. The environmental isolates show enhanced resistance to lysozyme as compared with the laboratory strains and this correlates with increased peptidoglycan acetylation. In strains that make a protein with an elongated linker, Rpf was bound to the cell wall, rather than being released to the growth medium, as occurs in reference strains. This rpf gene was introduced into a lysozyme-sensitive reference strain. Both rpf genes were expressed in transformants which showed a slight but statistically significant increase in lysozyme resistance. en
dc.format.extent 15 en
dc.language.iso eng
dc.relation.ispartof Microbial Ecology en
dc.title Structural changes and cellular localization of resuscitation promoting factor in environmental isolates of Micrococcus luteus en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1007/s00248-009-9573-1
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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