Show simple item record Wilkinson, Michael J. Caligari, Peter D. S. Dear, P. H. Vu, Thi-Ha-Giang 2011-06-01T07:43:51Z 2011-06-01T07:43:51Z 2010-02-08
dc.identifier.citation Wilkinson , M J , Caligari , P D S , Dear , P H & Vu , T-H-G 2010 , ' BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping ' PLoS One , vol 5 , no. 2 , pp. paper e9089 . DOI: 10.1371/journal.pone.0009089 en
dc.identifier.issn 1932-6203
dc.identifier.other PURE: 162664
dc.identifier.other PURE UUID: 912e8c30-ca9d-4b88-940b-c211ef6d8c27
dc.identifier.other dspace: 2160/6848
dc.identifier.other Ibers_20121112_1204.csv: row: 1005
dc.identifier.other Scopus: 77949412665
dc.identifier.other PubMed: 20161702
dc.identifier.other PubMedCentral: PMC2816996
dc.description Vu, G.T.H., Dear, P.H., Caligari, P.D.S., Wilkinson, M.J.* (2010). BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping.  PLoS One, 5, (2), paper e9089. IMPF: 03.56 Sponsorship: Biohybrids International and Sumatra Biosciences en
dc.description.abstract Physical and linkage mapping underpin efforts to sequence and characterize the genomes of eukaryotic organisms by providing a skeleton framework for whole genome assembly. Hitherto, linkage and physical 'contig' maps were generated independently prior to merging. Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline. Using Arabidopsis thaliana as an exemplar, a set of 40 Sequence Tagged Site (STS) markers spanning ~10% of chromosome 4 were simultaneously assembled onto a BAP map compiled using both a series of BAC pools each comprising 0.7x genome coverage and dilute (0.7x genome) samples of sheared genomic DNA. The resultant BAP map overcomes the need for polymorphic loci to separate genetic loci by recombination and allows physical mapping in segments of suppressed recombination that are difficult to analyze using traditional mapping techniques. Even virtual 'BAC-HAPPY-mapping' to convert BAC landing data into BAC linkage contigs is possible. en
dc.language.iso eng
dc.relation.ispartof PLoS One en
dc.rights en
dc.title BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.description.version publishersversion en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

Files in this item

Aside from theses and in the absence of a specific licence document on an item page, all works in Cadair are accessible under the CC BY-NC-ND Licence. AU theses and dissertations held on Cadair are made available for the purposes of private study and non-commercial research and brief extracts may be reproduced under fair dealing for the purpose of criticism or review. If you have any queries in relation to the re-use of material on Cadair, contact

This item appears in the following Collection(s)

Show simple item record

Search Cadair

Advanced Search