Show simple item record Michael J. en_US Peter D. S. en_US P. H. en_US Thi-Ha-Giang en_US 2011-06-01T07:43:51Z 2011-06-01T07:43:51Z 2010-02-08 en_US
dc.identifier en_US
dc.identifier.citation Wilkinson , M J , Caligari , P D S , Dear , P H & Vu , T-H-G 2010 , ' BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping ' PLoS One , vol 5 , no. 2 , pp. paper e9089 . , 10.1371/journal.pone.0009089 en_US
dc.identifier.other PURE: 162664 en_US
dc.identifier.other dspace: 2160/6848 en_US
dc.description.abstract Physical and linkage mapping underpin efforts to sequence and characterize the genomes of eukaryotic organisms by providing a skeleton framework for whole genome assembly. Hitherto, linkage and physical 'contig' maps were generated independently prior to merging. Here, we develop a new and easy method, BAC HAPPY MAPPING (BAP mapping), that utilizes BAC library pools as a HAPPY mapping panel together with an Mbp-sized DNA panel to integrate the linkage and physical mapping efforts into one pipeline. Using Arabidopsis thaliana as an exemplar, a set of 40 Sequence Tagged Site (STS) markers spanning ~10% of chromosome 4 were simultaneously assembled onto a BAP map compiled using both a series of BAC pools each comprising 0.7x genome coverage and dilute (0.7x genome) samples of sheared genomic DNA. The resultant BAP map overcomes the need for polymorphic loci to separate genetic loci by recombination and allows physical mapping in segments of suppressed recombination that are difficult to analyze using traditional mapping techniques. Even virtual 'BAC-HAPPY-mapping' to convert BAC landing data into BAC linkage contigs is possible. en_US
dc.relation.ispartof PLoS One en_US
dc.title BAC-HAPPY mapping (BAP mapping): a new and efficient protocol for physical mapping en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US

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