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dc.contributor.author Peter M. en_US
dc.contributor.author Andreas en_US
dc.contributor.author Jan P. en_US
dc.contributor.author Thomas en_US
dc.contributor.author Nils en_US
dc.contributor.author Takashi en_US
dc.contributor.author Thi-Ha-Giang en_US
dc.date.accessioned 2011-06-01T07:48:32Z
dc.date.available 2011-06-01T07:48:32Z
dc.date.issued 2010-11 en_US
dc.identifier http://dx.doi.org/10.1007/s10142-010-0173-4 en_US
dc.identifier.citation Chandler , P M , Graner , A , Buchmann , J P , Wicker , T , Stein , N , Matsumoto , T & Vu , T-H-G 2010 , ' Fine mapping and syntenic integration of the semi-dwarfing gene sdw3 of barley ' Functional and Integrative Genomics , vol 10 , no. 4 , pp. 509-521 . , 10.1007/s10142-010-0173-4 en_US
dc.identifier.other PURE: 162699 en_US
dc.identifier.other dspace: 2160/6849 en_US
dc.identifier.uri http://hdl.handle.net/2160/6849
dc.description.abstract The barley mutant allele sdw3 confers a gibberellin-insensitive, semi-dwarf phenotype with potential for breeding of new semi-dwarfed barley cultivars. Towards map-based cloning, sdw3 was delimited by high-resolution genetic mapping to a 0.04 cM interval in a “cold spot” of recombination of the proximal region of the short arm of barley chromosome 2H. Extensive synteny between the barley Sdw3 locus (Hvu_sdw3) and the orthologous regions (Osa_sdw3, Sbi_sdw3, Bsy_sdw3) of three other grass species (Oryza sativa, Sorghum bicolor, Brachypodium sylvaticum) allowed for efficient synteny-based marker saturation in the target interval. Comparative sequence analysis revealed colinearity for 23 out of the 38, 35, and 29 genes identified in Brachypodium, rice, and Sorghum, respectively. Markers co-segregating with Hvu_sdw3 were generated from two of these genes. Initial attempts at chromosome walking in barley were performed with seven orthologous gene probes which were delimiting physical distances of 223, 123, and 127 kb in Brachypodium, rice, and Sorghum, respectively. Six non-overlapping small bacterial artificial chromosome (BAC) clone contigs (cumulative length of 670 kb) were obtained, which indicated a considerably larger physical size of Hvu_sdw3. Low-pass sequencing of selected BAC clones from these barley contigs exhibited a substantially lower gene frequency per physical distance and the presence of additional non-colinear genes. Four candidate genes for sdw3 were identified within barley BAC sequences that either co-segregated with the gene sdw3 or were located adjacent to these co-segregating genes. Identification of genic sequences in the sdw3 context provides tools for marker-assisted selection. Eventual identification of the actual gene will contribute new information for a basic understanding of the mechanisms underlying growth regulation in barley. en_US
dc.format.extent 13 en_US
dc.relation.ispartof Functional and Integrative Genomics en_US
dc.title Fine mapping and syntenic integration of the semi-dwarfing gene sdw3 of barley en_US
dc.contributor.pbl Aberystwyth University en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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