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dc.contributor.author Melanie en_US
dc.contributor.author M. T. en_US
dc.contributor.author Glyn en_US
dc.contributor.author Dan en_US
dc.contributor.editor C. en_US
dc.date.accessioned 2011-06-01T08:56:44Z
dc.date.available 2011-06-01T08:56:44Z
dc.date.issued 2010-06-10 en_US
dc.identifier http://dx.doi.org/10.1007/978-90-481-8706-5_60 en_US
dc.identifier 9789048187058 en_US
dc.identifier.citation Febrer , M , Abberton , M T , Jenkins , G & Milbourne , D 2010 , ' Exploring the potential for translational genomics approaches in forage legumes: regions of highly conserved microsynteny between white clover and Medicago truncatula revealed by BAC sequencing ' . in C Huyghe (ed.) , Sustainable use of Genetic Diversity in Forage and Turf Breeding . Springer Science+Business Media , pp. 415-419 . , 10.1007/978-90-481-8706-5_60 en_US
dc.identifier.other PURE: 165663 en_US
dc.identifier.other dspace: 2160/6856 en_US
dc.identifier.uri http://hdl.handle.net/2160/6856
dc.description.abstract The model legume species Medicago truncatula is a potentially useful tool for gene discovery in white clover using translational genomics strategies. A prerequisite to the practical implementation of this approach is a good understanding of the extent of conservation of gene order between the species. Previous studies have demonstrated conservation at the macrosyntenic level, but no published information exists on the extent of conserved microsynteny between these species. In a previously published study, we reported the construction of a BAC library of white clover, the end sequencing of approximately 700 clover BACs, and the comparison of these BAC-end sequences to the M. truncatula genome. We found that 14 paired BAC-ends were shown to have the equivalent pairs of M. truncatula sequence on the same M. truncatula BAC clone or contig sequence within a span of 25?200 Kb, suggesting they represent orthologous regions in the two species. In this follow-up analysis, we have chosen five of these BACs, sequenced them to approximately six-fold coverage, and compared the resulting assembled contigs to their putatively equivalent regions of M. truncatula. Highly conserved gene content and almost complete conservation of gene order and orientation for all five sequences were found, suggesting that translational genomics approaches for gene discovery using Medicago could be successful. en_US
dc.format.extent 5 en_US
dc.publisher Springer Science+Business Media en_US
dc.relation.ispartof Sustainable use of Genetic Diversity in Forage and Turf Breeding en_US
dc.title Exploring the potential for translational genomics approaches in forage legumes: regions of highly conserved microsynteny between white clover and Medicago truncatula revealed by BAC sequencing en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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