Metabolite fingerprinting of urine suggests breed-specific dietary metabolism differences in domestic dogs

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dc.contributor.author Scott, Ian M.
dc.contributor.author Jones, P. G.
dc.contributor.author Allaway, David
dc.contributor.author Draper, John
dc.contributor.author Beckmann, Manfred
dc.contributor.author Overy, David P.
dc.contributor.author Enot, David P.
dc.date.accessioned 2011-06-01T09:17:52Z
dc.date.available 2011-06-01T09:17:52Z
dc.date.issued 2011-06-01
dc.identifier.citation Scott , I M , Jones , P G , Allaway , D , Draper , J , Beckmann , M , Overy , D P & Enot , D P 2011 , ' Metabolite fingerprinting of urine suggests breed-specific dietary metabolism differences in domestic dogs ' Unknown Journal , pp. 1127-1138 . en
dc.identifier.other PURE: 171941
dc.identifier.other dspace: 2160/6861
dc.identifier.uri http://hdl.handle.net/2160/6861
dc.description Beckmann, M., Enot, D.P., Overy, D.P., Scott, I.M., Jones, P.G., Allaway, D., Draper, J. (2010). Metabolite fingerprinting of urine suggests breed-specific dietary metabolism differences in domestic dogs.   British Journal of Nutrition, 103, (8), 1127-1138. en
dc.description.abstract Selective breeding of dogs has culminated in a large number of modern breeds distinctive in terms of size, shape and behaviour. Inadvertently, a range of breed-specific genetic disorders have become fixed in some pure-bred populations. Several inherited conditions confer chronic metabolic defects that are influenced strongly by diet, but it is likely that many less obvious breed-specific differences in physiology exist. Using Labrador retrievers and miniature Schnauzers maintained in a simulated domestic setting on a controlled diet, an experimental design was validated in relation to husbandry, sampling and sample processing for metabolomics. Metabolite fingerprints were generated from ¿spot¿ urine samples using flow injection electrospray MS (FIE-MS). With class based on breed, urine chemical fingerprints were modelled using Random Forest (a supervised data classification technique), and metabolite features (m/z) explanatory of breed-specific differences were putatively annotated using the ARMeC database (http://www.armec.org). GC-MS profiling to confirm FIE-MS predictions indicated major breed-specific differences centred on the metabolism of diet-related polyphenols. Metabolism of further diet components, including potentially prebiotic oligosaccharides, animal-derived fats and glycerol, appeared significantly different between the two breeds. Analysis of the urinary metabolome of young male dogs representative of a wider range of breeds from animals maintained under domestic conditions on unknown diets provided preliminary evidence that many breeds may indeed have distinctive metabolic differences, with significant differences particularly apparent in comparisons between large and smaller breeds. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title Metabolite fingerprinting of urine suggests breed-specific dietary metabolism differences in domestic dogs en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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