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dc.contributor.author C. M. en_US
dc.contributor.author A. G. S. en_US
dc.contributor.author Colin John en_US
dc.contributor.author J. G. L. en_US
dc.contributor.author R. C. en_US
dc.contributor.author J. E. en_US
dc.contributor.author Stephen Michael en_US
dc.contributor.author Iain Simon en_US
dc.contributor.author D. E. en_US
dc.contributor.author S. L. en_US
dc.date.accessioned 2011-06-01T12:48:09Z
dc.date.available 2011-06-01T12:48:09Z
dc.date.issued 2010-06 en_US
dc.identifier http://dx.doi.org/10.1016/j.biortech.2010.01.084 en_US
dc.identifier.citation Martel , C M , Warrilow , A G S , Jackson , C J , Mullins , J G L , Togawa , R C , Parker , J E , Morris , S M , Donnison , I S , Kelly , D E & Kelly , S L 2010 , ' Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans ' Bioresource Technology , vol 101 , no. 12 , pp. 4395-4402 . , 10.1016/j.biortech.2010.01.084 en_US
dc.identifier.other PURE: 164699 en_US
dc.identifier.other dspace: 2160/6873 en_US
dc.identifier.uri http://hdl.handle.net/2160/6873
dc.description.abstract Microbial inulinases find application in food, pharmaceutical and biofuel industries. Here, a novel Lactobacillus paracasei β-fructosidase was overexpressed as truncated cytosolic protein (tfosEp) in Escherichia coli. Purified tfosEp was thermostable (10–50 °C) with a pH optimum of 5; it showed highest affinity for bacterial levan (β[2-6] linked fructose) followed by nystose, chicory inulin, 1-kestose (β[2-1] linkages) and sucrose (Km values of 0.5, 15, 15.6, 49 and 398 mM, respectively). Hydrolysis of polyfructose moieties in agriculturally-sourced grass juice (GJ) with tfosEp resulted in the release of >13 mg/ml more bioavailable fructose than was measured in untreated GJ. Bioethanol yields from fermentation experiments with Brewer’s yeast and GJ + tfosEp were >25% higher than those achieved using untreated GJ feedstock (36.5[±4.3] and 28.2[±2.7] mg ethanol/ml, respectively). This constitutes the first specific study of the potential to ferment ethanol from grass juice and the utility of a novel core domain of β-fructosidase from L. paracasei. en_US
dc.format.extent 8 en_US
dc.relation.ispartof Bioresource Technology en_US
dc.subject Bioethanol en_US
dc.subject Fructan en_US
dc.subject Fructanase en_US
dc.subject Lactobacillus paracasei en_US
dc.subject Perennial ryegrass en_US
dc.title Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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