Show simple item record Martel, C. M. Warrilow, A. G. S. Jackson, Colin John Mullins, J. G. L. Togawa, R. C. Parker, J. E. Morris, Stephen Michael Donnison, Iain Simon Kelly, Diane Elizabeth Kelly, Steven Lewis 2011-06-01T12:48:09Z 2011-06-01T12:48:09Z 2010-06
dc.identifier.citation Martel , C M , Warrilow , A G S , Jackson , C J , Mullins , J G L , Togawa , R C , Parker , J E , Morris , S M , Donnison , I S , Kelly , D E & Kelly , S L 2010 , ' Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans ' Bioresource Technology , vol 101 , no. 12 , pp. 4395-4402 . DOI: 10.1016/j.biortech.2010.01.084 en
dc.identifier.issn 0960-8524
dc.identifier.other PURE: 164699
dc.identifier.other PURE UUID: 79ae6510-bf10-40dc-90fb-07ddcfa80f3e
dc.identifier.other dspace: 2160/6873
dc.identifier.other DSpace_20121128.csv: row: 4212
dc.identifier.other IBERS: 0000018648
dc.identifier.other Ibers_20121112_1204.csv: row: 914
dc.identifier.other RAD: 1794
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 966
dc.identifier.other RAD: 1062
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 680
dc.identifier.other RAD: 5087
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 2130
dc.identifier.other Scopus: 77949264180
dc.identifier.other PubMed: 20153640
dc.description Martel, C.M., Warrilow, A.G.S., Jackson, C.O., Mullins, J.G.L., Togawa, R.C., Parker, J.E., Morris, S.M., Donnison, I.S., Kelly, D.E., Kelly, S.L. (2010). Expression, purification and use of Lactobacillus paracasei beta fructosidase to optimise production of bioethanol from grass biomass.   Bioresource Technology, 101, (12), 4395-4402. IMPF: 04.36 Sponsorship: Welsh Energy Research Centre (WERC, UK)/European Regional Development Fund of the EU; BBSRC RONO: 03134;06321; BBS/E/W/00003134B; BBS/E/W/00003134E en
dc.description.abstract Microbial inulinases find application in food, pharmaceutical and biofuel industries. Here, a novel Lactobacillus paracasei β-fructosidase was overexpressed as truncated cytosolic protein (tfosEp) in Escherichia coli. Purified tfosEp was thermostable (10–50 °C) with a pH optimum of 5; it showed highest affinity for bacterial levan (β[2-6] linked fructose) followed by nystose, chicory inulin, 1-kestose (β[2-1] linkages) and sucrose (Km values of 0.5, 15, 15.6, 49 and 398 mM, respectively). Hydrolysis of polyfructose moieties in agriculturally-sourced grass juice (GJ) with tfosEp resulted in the release of >13 mg/ml more bioavailable fructose than was measured in untreated GJ. Bioethanol yields from fermentation experiments with Brewer’s yeast and GJ + tfosEp were >25% higher than those achieved using untreated GJ feedstock (36.5[±4.3] and 28.2[±2.7] mg ethanol/ml, respectively). This constitutes the first specific study of the potential to ferment ethanol from grass juice and the utility of a novel core domain of β-fructosidase from L. paracasei. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Bioresource Technology en
dc.rights en
dc.subject Bioethanol en
dc.subject Fructan en
dc.subject Fructanase en
dc.subject Lactobacillus paracasei en
dc.subject Perennial ryegrass en
dc.title Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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