Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans

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dc.contributor.author Martel, C. M.
dc.contributor.author Warrilow, A. G. S.
dc.contributor.author Jackson, Colin John
dc.contributor.author Mullins, J. G. L.
dc.contributor.author Togawa, R. C.
dc.contributor.author Parker, J. E.
dc.contributor.author Morris, Stephen Michael
dc.contributor.author Donnison, Iain Simon
dc.contributor.author Kelly, D. E.
dc.contributor.author Kelly, S. L.
dc.date.accessioned 2011-06-01T12:48:09Z
dc.date.available 2011-06-01T12:48:09Z
dc.date.issued 2010-06
dc.identifier.citation Martel , C M , Warrilow , A G S , Jackson , C J , Mullins , J G L , Togawa , R C , Parker , J E , Morris , S M , Donnison , I S , Kelly , D E & Kelly , S L 2010 , ' Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans ' Bioresource Technology , vol 101 , no. 12 , pp. 4395-4402 . en
dc.identifier.issn 0960-8524
dc.identifier.other PURE: 164699
dc.identifier.other dspace: 2160/6873
dc.identifier.uri http://hdl.handle.net/2160/6873
dc.description Martel, C.M., Warrilow, A.G.S., Jackson, C.O., Mullins, J.G.L., Togawa, R.C., Parker, J.E., Morris, S.M., Donnison, I.S., Kelly, D.E., Kelly, S.L. (2010). Expression, purification and use of Lactobacillus paracasei beta fructosidase to optimise production of bioethanol from grass biomass.   Bioresource Technology, 101, (12), 4395-4402. IMPF: 04.36 RONO: 03134;06321 Sponsorship: Welsh Energy Research Centre (WERC, UK)/European Regional Development Fund of the EU en
dc.description.abstract Microbial inulinases find application in food, pharmaceutical and biofuel industries. Here, a novel Lactobacillus paracasei β-fructosidase was overexpressed as truncated cytosolic protein (tfosEp) in Escherichia coli. Purified tfosEp was thermostable (10–50 °C) with a pH optimum of 5; it showed highest affinity for bacterial levan (β[2-6] linked fructose) followed by nystose, chicory inulin, 1-kestose (β[2-1] linkages) and sucrose (Km values of 0.5, 15, 15.6, 49 and 398 mM, respectively). Hydrolysis of polyfructose moieties in agriculturally-sourced grass juice (GJ) with tfosEp resulted in the release of >13 mg/ml more bioavailable fructose than was measured in untreated GJ. Bioethanol yields from fermentation experiments with Brewer’s yeast and GJ + tfosEp were >25% higher than those achieved using untreated GJ feedstock (36.5[±4.3] and 28.2[±2.7] mg ethanol/ml, respectively). This constitutes the first specific study of the potential to ferment ethanol from grass juice and the utility of a novel core domain of β-fructosidase from L. paracasei. en
dc.format.extent 8 en
dc.language.iso eng
dc.relation.ispartof Bioresource Technology en
dc.subject Bioethanol en
dc.subject Fructan en
dc.subject Fructanase en
dc.subject Lactobacillus paracasei en
dc.subject Perennial ryegrass en
dc.title Expression, purification and use of the soluble domain of Lactobacillus paracasei β-fructosidase to optimise production of bioethanol from grass fructans en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.biortech.2010.01.084
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.contributor.institution Institute of Geography & Earth Sciences en
dc.description.status Peer reviewed en


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