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dc.contributor.author Marcia M. de O. en_US
dc.contributor.author Tim en_US
dc.contributor.author Barbara Dorothea en_US
dc.contributor.author Susan Jane en_US
dc.contributor.author Emma Jane en_US
dc.contributor.author Phillip en_US
dc.date.accessioned 2011-06-01T13:04:34Z
dc.date.available 2011-06-01T13:04:34Z
dc.date.issued 2010-04 en_US
dc.identifier http://dx.doi.org/10.1111/j.1467-7652.2009.00485.x en_US
dc.identifier.citation Buanafina , M M D O , Langdon , T , Hauck , B D , Dalton , S J , Timms-Taravella , E J & Morris , P 2010 , ' Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea) ' Plant Biotechnology Journal , vol 8 , no. 3 , pp. 316-331 . , 10.1111/j.1467-7652.2009.00485.x en_US
dc.identifier.other PURE: 163059 en_US
dc.identifier.other dspace: 2160/6877 en_US
dc.identifier.uri http://hdl.handle.net/2160/6877
dc.description.abstract In the cell walls of grasses, ferulic acid is esterified to arabinoxylans and undergoes oxidative reactions to form ferulates dimers, trimers and oligomers. Feruloylation of arabinoxylan is considered important not only because it leads to cross-linked xylans but also because ferulates may act as a nucleating site for the formation of lignin and hence link arabinoxylans to lignin by forming a lignin–ferulate–arabinoxylan complex. Such cross-linking is among the main factors inhibiting the release of fermentable carbohydrates from grasses either for ruminant nutrition or for biofuel production. We have found that significant reductions in the levels of monomeric and dimeric phenolics can be achieved in the growing cell walls during plant development in leaves of Festuca arundinacea by constitutive intracellular targeted expression of Aspergillus niger ferulic acid esterase (FAEA). We propose that this occurred by directly disrupting ester bonds linking phenolics to cell wall polysaccharides by apoplast targeting or by preventing excessive feruloylation of cell wall carbohydrates prior to their incorporation into the cell wall, by targeting to the Golgi membrane system. Plants with lower cell wall ferulate levels, which showed increased digestibility and increased rates of cellulase-mediated release of fermentable sugars, were identified. Targeting FAE to the Golgi was found to be more effective than targeting to the ER, which supports the current theories of the Golgi as the site of feruloylation of arabinoxylans. It is concluded that targeting FAEA expression to the Golgi or apoplast is likely to be an effective strategy for improving wall digestibility in grass species used for fodder or cellulosic ethanol production. en_US
dc.format.extent 16 en_US
dc.relation.ispartof Plant Biotechnology Journal en_US
dc.subject cell wall en_US
dc.subject ferulic acid esterase en_US
dc.subject transgenic grass en_US
dc.subject hydroxycinnamic acids en_US
dc.subject cross-linking en_US
dc.subject deigestibility en_US
dc.subject tall fescue en_US
dc.title Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea) en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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