Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea)

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dc.contributor.author Buanafina, Marcia M. de O.
dc.contributor.author Langdon, Tim
dc.contributor.author Hauck, Barbara Dorothea
dc.contributor.author Dalton, Susan Jane
dc.contributor.author Timms-Taravella, Emma Jane
dc.contributor.author Morris, Phillip
dc.date.accessioned 2011-06-01T13:04:34Z
dc.date.available 2011-06-01T13:04:34Z
dc.date.issued 2010-04
dc.identifier.citation Buanafina , M M D O , Langdon , T , Hauck , B D , Dalton , S J , Timms-Taravella , E J & Morris , P 2010 , ' Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea) ' Plant Biotechnology Journal , vol 8 , no. 3 , pp. 316-331 . en
dc.identifier.issn 1467-7644
dc.identifier.other PURE: 163059
dc.identifier.other dspace: 2160/6877
dc.identifier.uri http://hdl.handle.net/2160/6877
dc.description Buanafina, M. M. . O., Langdon, T., Hauck, B., Dalton, S. J., Timms-Taravella, E., Morris, P. (2010). Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea). Plant Biotechnology Journal, 8 (3), 316-331. Sponsorship: BBSRC/Genencor Inc. IMPF: 04.88 en
dc.description.abstract In the cell walls of grasses, ferulic acid is esterified to arabinoxylans and undergoes oxidative reactions to form ferulates dimers, trimers and oligomers. Feruloylation of arabinoxylan is considered important not only because it leads to cross-linked xylans but also because ferulates may act as a nucleating site for the formation of lignin and hence link arabinoxylans to lignin by forming a lignin–ferulate–arabinoxylan complex. Such cross-linking is among the main factors inhibiting the release of fermentable carbohydrates from grasses either for ruminant nutrition or for biofuel production. We have found that significant reductions in the levels of monomeric and dimeric phenolics can be achieved in the growing cell walls during plant development in leaves of Festuca arundinacea by constitutive intracellular targeted expression of Aspergillus niger ferulic acid esterase (FAEA). We propose that this occurred by directly disrupting ester bonds linking phenolics to cell wall polysaccharides by apoplast targeting or by preventing excessive feruloylation of cell wall carbohydrates prior to their incorporation into the cell wall, by targeting to the Golgi membrane system. Plants with lower cell wall ferulate levels, which showed increased digestibility and increased rates of cellulase-mediated release of fermentable sugars, were identified. Targeting FAE to the Golgi was found to be more effective than targeting to the ER, which supports the current theories of the Golgi as the site of feruloylation of arabinoxylans. It is concluded that targeting FAEA expression to the Golgi or apoplast is likely to be an effective strategy for improving wall digestibility in grass species used for fodder or cellulosic ethanol production. en
dc.format.extent 16 en
dc.language.iso eng
dc.relation.ispartof Plant Biotechnology Journal en
dc.subject cell wall en
dc.subject ferulic acid esterase en
dc.subject transgenic grass en
dc.subject hydroxycinnamic acids en
dc.subject cross-linking en
dc.subject deigestibility en
dc.subject tall fescue en
dc.title Targeting expression of a fungal ferulic acid esterase to the apoplast, endoplasmic reticulum or golgi can disrupt feruloylation of the growing cell wall and increase the biodegradability of tall fescue (Festuca arundinacea) en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1111/j.1467-7652.2009.00485.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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