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dc.contributor.author Yamaguchi, Takahiro
dc.contributor.author Rose, Michael T.
dc.contributor.author Miyazawa, K.
dc.contributor.author Tanaka, Sachi
dc.contributor.author Watanabe, Hitoshi
dc.contributor.author Kanaya, T.
dc.contributor.author Takakura, Ikuro
dc.contributor.author al, et
dc.contributor.author Katamine, Shigeru
dc.contributor.author Kitazawa, Haruki
dc.contributor.author Hondo, Tetsuya
dc.date.accessioned 2011-06-06T10:33:51Z
dc.date.available 2011-06-06T10:33:51Z
dc.date.issued 2011-06-06
dc.identifier.citation Yamaguchi , T , Rose , M T , Miyazawa , K , Tanaka , S , Watanabe , H , Kanaya , T , Takakura , I , al , Katamine , S , Kitazawa , H & Hondo , T 2011 , ' Transcytosis of murine-adapted Bovine Spongiform Encephalopathy agents in an in vitro bovine M cell model ' Unknown Journal , pp. 12285-12291 . en
dc.identifier.other PURE: 174062
dc.identifier.other dspace: 2160/6924
dc.identifier.uri http://hdl.handle.net/2160/6924
dc.description Miyazawa, K., Kanaya, T., Takakura, I., Tanaka, S., Hondo, T., Watanabe, H., Rose, M. T., Kitazawa, H., Yamaguchi, T., Katamine, S., Nishida, N., Aso, H. (2010). Transcytosis of murine-adapted Bovine Spongiform Encephalopathy agents in an in vitro bovine M cell model.   Journal of Virology, 84, 12285-12291. en
dc.description.abstract Transmissible spongiform encephalopathies (TSE), including bovine spongiform encephalopathy (BSE), are fatal neurodegenerative disorders in humans and animals. BSE appears to have spread to cattle through the consumption of feed contaminated with BSE/scrapie agents. In the case of an oral infection, the agents have to cross the gut-epithelial barrier. We recently established a bovine intestinal epithelial cell line (BIE cells) that can differentiate into the M cell type in vitro after lymphocytic stimulation (K. Miyazawa, T. Hondo, T. Kanaya, S. Tanaka, I. Takakura, W. Itani, M. T. Rose, H. Kitazawa, T. Yamaguchi, and H. Aso, Histochem. Cell Biol. 133:125-134, 2010). In this study, we evaluated the role of M cells in the intestinal invasion of the murine-adapted BSE (mBSE) agent using our in vitro bovine intestinal epithelial model. We demonstrate here that M cell-differentiated BIE cells are able to transport the mBSE agent without inactivation at least 30-fold more efficiently than undifferentiated BIE cells in our in vitro model. As M cells in the follicle-associated epithelium are known to have a high ability to transport a variety of macromolecules, viruses, and bacteria from gut lumen to mucosal immune cells, our results indicate the possibility that bovine M cells are able to deliver agents of TSE, not just the mBSE agent. en
dc.format.extent 7 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title Transcytosis of murine-adapted Bovine Spongiform Encephalopathy agents in an in vitro bovine M cell model en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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