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dc.contributor.author Nash, Deborah M.
dc.contributor.author Lane, Elizabeth A.
dc.contributor.author Sheldon, I. Martin
dc.contributor.author Herath, Shan
dc.date.accessioned 2011-06-06T11:15:24Z
dc.date.available 2011-06-06T11:15:24Z
dc.date.issued 2011-06-06
dc.identifier.citation Nash , D M , Lane , E A , Sheldon , I M & Herath , S 2011 , ' Endometrial explant culture to study the response of equine endometrium to insemination ' Domestic Animals , pp. 670-676 . en
dc.identifier.other PURE: 172895
dc.identifier.other dspace: 2160/6929
dc.identifier.uri http://hdl.handle.net/2160/6929
dc.description Nash, D. M., Sheldon, I. M., Herath, S., Lane, E. A. (2010). Endometrial explant culture to study the response of equine endometrium to insemination.   Reproduction in Domestic Animals, 45, (4), 670-676. en
dc.description.abstract Mating-induced endometritis (MIE) is ubiquitous in the horse after natural mating and artificial insemination with frozen/thawed semen causing the most aggressive response. The majority of mares eliminate MIE 24¿48 h after insemination. An endometrial explant culture was tested as a potential in vitro exemplar for sperm-induced MIE. Endometrial prostaglandin F2¿ (PGF2¿) secretion and expression of interleukin-8 (IL-8) were used as markers of inflammation. Endometrial explants were cultured from uteri collected from follicular phase mares. Explants were challenged with 1 or 10 × 106 sperm/ml frozen/thawed semen, chilled semen, washed sperm or seminal plasma. Medium was collected 24 and 72 h after challenge and assayed for PGF2¿ by radioimmunoassay. Treatment of endometrial explants with frozen/thawed, chilled semen or washed sperm did not change the secretion of PGF2¿ compared with untreated controls. However, 24 h after challenge cultured explants expressed IL-8. The in vitro endometrial explant system did not represent the in vivo response to semen when PGF2¿ was used as a marker of inflammation, yet the use of gene expression as an inflammatory marker warrants further investigation. en
dc.format.extent 7 en
dc.language.iso eng
dc.relation.ispartof Domestic Animals en
dc.title Endometrial explant culture to study the response of equine endometrium to insemination en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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