Accumulation of chlorophyll catabolites photosensitizes the hypersensitive response elicited by Pseudomonas syringae in Arabidopsis

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dc.contributor.author Mur, Luis Alejandro Jose
dc.contributor.author Aubry, Sylvain
dc.contributor.author Mondhe, Madhav
dc.contributor.author Kingston-Smith, Alison Helen
dc.contributor.author Gallagher, Joe
dc.contributor.author Timms-Taravella, Emma Jane
dc.contributor.author James, Caron Louise
dc.contributor.author Papp, Istvan
dc.contributor.author Hörtensteiner, Stefan
dc.contributor.author Thomas, Howard
dc.contributor.author Ougham, Helen Joan
dc.date.accessioned 2011-06-07T08:44:31Z
dc.date.available 2011-06-07T08:44:31Z
dc.date.issued 2010-08-05
dc.identifier.citation Mur , L A J , Aubry , S , Mondhe , M , Kingston-Smith , A H , Gallagher , J , Timms-Taravella , E J , James , C L , Papp , I , Hörtensteiner , S , Thomas , H & Ougham , H J 2010 , ' Accumulation of chlorophyll catabolites photosensitizes the hypersensitive response elicited by Pseudomonas syringae in Arabidopsis ' New Phytologist , vol 188 , no. 1 , pp. 161-174 . , 10.1111/j.1469-8137.2010.03377.x en
dc.identifier.issn 0028-646X
dc.identifier.other PURE: 163862
dc.identifier.other dspace: 2160/6947
dc.identifier.uri http://hdl.handle.net/2160/6947
dc.identifier.uri http://onlinelibrary.wiley.com/doi/10.1111/j.1469-8137.2010.03377.x/abstract en
dc.description Mur, L. A. J., Aubry, S., Mondhe, M., Kingston-Smith, A. H., Gallagher, J. A., Timms-Taravella, E., James, C. L., Papp, I., Hortensteiner, S., Thomas, Howard, Ougham, H. J. (2010). Accumulation of chlorophyll catabolites photosensitizes the hypersensitive response elicited by Pseudomonas syringae in Arabidopsis.   New Phytologist, 188 (1), 161-174. IMPF: 06.51 Sponsorship: Biotechnology and Biological Sciences Research Council (BBSRC) en
dc.description.abstract The staygreen (SGR) gene encodes a chloroplast-targeted protein which promotes chlorophyll degradation via disruption of light-harvesting complexes (LHCs). Over-expression of SGR in Arabidopsis (SGR-OX) in a Columbia-0 (Col-0) background caused spontaneous necrotic flecking. To relate this to the hypersensitive response (HR), Col-0, SGR-OX and RNAi SGR (SGRi) lines were challenged with Pseudomonas syringae pv tomato (Pst) encoding the avirulence gene avrRpm1. Increased and decreased SGR expression, respectively, accelerated and suppressed the kinetics of HR-cell death. In Col-0, SGR transcript increased at 6 h after inoculation (hai) when tissue electrolyte leakage indicated the initiation of cell death. Excitation of the chlorophyll catabolite pheophorbide (Pheide) leads to the formation of toxic singlet oxygen (1O2). Pheide was first detected at 6 hai with Pst avrRpm1 and was linked to 1O2 generation and correlated with reduced Pheide a oxygenase (PaO) protein concentrations. The maximum quantum efficiency of photosystem II (Fv/Fm), quantum yield of electron transfer at photosystem II (φPSII), and photochemical quenching (qP) decreased at 6 hai in Col-0 but not in SGRi. Disruption of photosynthetic electron flow will cause light-dependent H2O2 generation at 6 hai. We conclude that disruption of LHCs, possibly influenced by SGR, and absence of PaO produce phototoxic chlorophyll catabolites and oxidative stress leading to the HR. en
dc.format.extent 14 en
dc.language.iso eng
dc.relation.ispartof New Phytologist en
dc.subject Arabidopsis en
dc.subject death en
dc.subject hypersensitive response en
dc.subject light-dependent pheophorbide en
dc.subject photosynthesis en
dc.subject Pseudomonas syringae en
dc.subject singlet oxygen (1O2) en
dc.subject staygreen en
dc.title Accumulation of chlorophyll catabolites photosensitizes the hypersensitive response elicited by Pseudomonas syringae in Arabidopsis en
dc.type Text en
dc.type.publicationtype Special issue (Journal) en
dc.identifier.doi http://dx.doi.org/10.1111/j.1469-8137.2010.03377.x
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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