Show simple item record Wilcockson, David C. Zhang, Lin Hastings, Michael H. Kyriacou, Charalambos P. Webster, Simon G. 2011-06-07T10:31:24Z 2011-06-07T10:31:24Z 2011-02-15
dc.identifier.citation Wilcockson , D C , Zhang , L , Hastings , M H , Kyriacou , C P & Webster , S G 2011 , ' A novel form of pigment-dispersing hormone in the central nervous system of the intertidal marine isopod, Eurydice pulchra (leach) ' Journal of Comparative Neurology , vol 519 , no. 3 , pp. 562-575 . DOI: 10.1002/cne.22533 en
dc.identifier.issn 0021-9967
dc.identifier.other PURE: 166525
dc.identifier.other PURE UUID: 2dbe54c9-791c-4622-ab2d-62f94658cb9f
dc.identifier.other dspace: 2160/6992
dc.identifier.other Ibers_20121112_1204.csv: row: 1256
dc.identifier.other RAD: 1070
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 688
dc.identifier.other RAD: 9423
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 2863
dc.identifier.other crossref: 10.1002/cne.22533
dc.identifier.other RAD: 1072
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 690
dc.identifier.other Scopus: 78650635865
dc.identifier.other PubMed: 21192084
dc.description IMPF: 03.80 en
dc.description.abstract Pigment-dispersing factor (PDF) is well known as a circadian clock output factor, which drives daily activity rhythms in many insects. The role of its homologue, pigment-dispersing hormone (PDH), in the regulation of circadian and/or circatidal rhythmicity in crustaceans is, however, poorly understood. The intertidal isopod crustacean, Eurydice pulchra has well-defined circatidal (12.4-hour) activity rhythms. In this study we show that this runs parallel to a circadian (24-hour) cycle of chromatophore dispersion. As a first step in determining the potential role of PDH in these rhythms, we have identified a novel form of PDH expressed in this species. Because conventional homology cloning was unsuccessful, we employed immuno-identification and Edman microsequencing to determine the primary structure of this peptide. From this, cDNA cloning identified the nucleotide encoding sequence and thus facilitated description of PDH neurons by in situ hybridization and immunohistochemistry. We show them to be morphologically similar to those that co-ordinate circadian activity rhythms in insects. In animals expressing both tidal (activity) and circadian (chromatophore) rhythms, however, there was no evidence for a corresponding periodicity in the expression of pdh transcript, as determined by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) in Eurydice heads. It is therefore suggested that any role for PDH in daily/tidal timing in Eurydice is not mediated at the transcriptional level, rather rhythms in neurohemal release may be important in such co-ordination. en
dc.format.extent 14 en
dc.language.iso eng
dc.relation.ispartof Journal of Comparative Neurology en
dc.rights en
dc.subject Indexing terms en
dc.subject pigment-dispersing hormone en
dc.subject circadian rhythms en
dc.subject isopod en
dc.subject Eurydice pulchra en
dc.subject Gene expression en
dc.subject in situ hybridization en
dc.subject immunohistochemistry en
dc.subject quantitative RT-PCR en
dc.title A novel form of pigment-dispersing hormone in the central nervous system of the intertidal marine isopod, Eurydice pulchra (leach) en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search Cadair

Advanced Search