dc.contributor.author |
Zhan, Bin |
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dc.contributor.author |
Perally, Samirah |
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dc.contributor.author |
Brophy, Peter M. |
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dc.contributor.author |
Xue, Jian |
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dc.contributor.author |
Goud, Gaddam |
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dc.contributor.author |
Liu, Sen |
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dc.contributor.author |
Deumic, Vehid |
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dc.contributor.author |
de Oliveira, Luciana |
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dc.contributor.author |
Bethony, Jeffrey |
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dc.contributor.author |
Bottazzi, Maria Elena |
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dc.contributor.author |
Jiang, Desheng |
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dc.contributor.author |
Gillespie, Portia |
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dc.contributor.author |
Xiao, Shu-hua |
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dc.contributor.author |
Gupta, Richi |
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dc.contributor.author |
Loukas, Alex |
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dc.contributor.author |
Ranjit, Najju |
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dc.contributor.author |
Lustigman, Sara |
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dc.contributor.author |
Hotez, Peter |
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dc.contributor.author |
Oksov, Yelena |
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dc.date.accessioned |
2011-06-09T09:01:45Z |
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dc.date.available |
2011-06-09T09:01:45Z |
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dc.date.issued |
2010-04 |
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dc.identifier.citation |
Zhan , B , Perally , S , Brophy , P M , Xue , J , Goud , G , Liu , S , Deumic , V , de Oliveira , L , Bethony , J , Bottazzi , M E , Jiang , D , Gillespie , P , Xiao , S , Gupta , R , Loukas , A , Ranjit , N , Lustigman , S , Hotez , P & Oksov , Y 2010 , ' Molecular Cloning, Biochemical Characterization, and Partial Protective Immunity of the Heme-Binding Glutathione S-Transferases from the Human Hookworm Necator americanus ' Infection and Immunity , vol 78 , no. 4 , pp. 1552-1563 . DOI: 10.1128/IAI.00848-09 |
en |
dc.identifier.issn |
0019-9567 |
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dc.identifier.other |
PURE: 164215 |
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dc.identifier.other |
PURE UUID: 882e6d20-3403-4bc5-9606-3e72ecf5a841 |
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dc.identifier.other |
dspace: 2160/7016 |
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dc.identifier.other |
Ibers_20121112_1204.csv: row: 1030 |
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dc.identifier.other |
RAD: 1754 |
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dc.identifier.other |
RAD_Outputs_All_ID_Import_20121105.csv: row: 932 |
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dc.identifier.other |
Scopus: 77950278221 |
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dc.identifier.other |
PubMed: 20145100 |
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dc.identifier.other |
PubMedCentral: PMC2849424 |
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dc.identifier.uri |
http://hdl.handle.net/2160/7016 |
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dc.identifier.uri |
http://iai.asm.org/cgi/reprint/IAI.00848-09v1 |
en |
dc.description |
Zhan, B., Perally, S., Brophy, P. M., Xue, J., Goud, G., Liu, S., Deumic, V., de Oliveira, L., Bethony, J., Bottazzi, M. E., Jiang, D., Gillespie, P., Xiao, S., Gupta, R., Loukas, A., Ranjit, N., Lustigman, S., Hotez, P. (2010). Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione transferases from the human hookworm Necator americanus. Infection and Immunity, 78 (4), 1552-1563. IMPF: 04.09 |
en |
dc.description.abstract |
Hookworm glutathione S-transferases (GSTs) are critical for parasite blood feeding and survival and represent potential targets for vaccination. Three cDNAs, each encoding a full-length GST protein from the human hookworm Necator americanus (and designated Na-GST-1, Na-GST-2, and Na-GST-3, respectively) were isolated from cDNA based on their sequence similarity to Ac-GST-1, a GST from the dog hookworm Ancylostoma caninum. The open reading frames of the three N. americanus GSTs each contain 206 amino acids with 51% to 69% sequence identity between each other and Ac-GST-1. Sequence alignment with GSTs from other organisms shows that the three Na-GSTs belong to a nematode-specific nu-class GST family. All three Na-GSTs, when expressed in Pichia pastoris, exhibited low lipid peroxidase and glutathione-conjugating enzymatic activities but high heme-binding capacities, and they may be involved in the detoxification and/or transport of heme. In two separate vaccine trials, recombinant Na-GST-1 formulated with Alhydrogel elicited 32 and 39% reductions in adult hookworm burdens (P < 0.05) following N. americanus larval challenge relative to the results for a group immunized with Alhydrogel alone. In contrast, no protection was observed in vaccine trials with Na-GST-2 or Na-GST-3. On the basis of these and other preclinical data, Na-GST-1 is under possible consideration for further vaccine development. |
en |
dc.format.extent |
12 |
en |
dc.language.iso |
eng |
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dc.relation.ispartof |
Infection and Immunity |
en |
dc.rights |
|
en |
dc.title |
Molecular Cloning, Biochemical Characterization, and Partial Protective Immunity of the Heme-Binding Glutathione S-Transferases from the Human Hookworm Necator americanus |
en |
dc.type |
/dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article |
en |
dc.identifier.doi |
http://dx.doi.org/10.1128/IAI.00848-09 |
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dc.contributor.institution |
Institute of Biological, Environmental and Rural Sciences |
en |
dc.description.status |
Peer reviewed |
en |