Show simple item record Zhan, Bin Perally, Samirah Brophy, Peter M. Xue, Jian Goud, Gaddam Liu, Sen Deumic, Vehid de Oliveira, Luciana Bethony, Jeffrey Bottazzi, Maria Elena Jiang, Desheng Gillespie, Portia Xiao, Shu-hua Gupta, Richi Loukas, Alex Ranjit, Najju Lustigman, Sara Hotez, Peter Oksov, Yelena 2011-06-09T09:01:45Z 2011-06-09T09:01:45Z 2010-04
dc.identifier.citation Zhan , B , Perally , S , Brophy , P M , Xue , J , Goud , G , Liu , S , Deumic , V , de Oliveira , L , Bethony , J , Bottazzi , M E , Jiang , D , Gillespie , P , Xiao , S , Gupta , R , Loukas , A , Ranjit , N , Lustigman , S , Hotez , P & Oksov , Y 2010 , ' Molecular Cloning, Biochemical Characterization, and Partial Protective Immunity of the Heme-Binding Glutathione S-Transferases from the Human Hookworm Necator americanus ' Infection and Immunity , vol 78 , no. 4 , pp. 1552-1563 . DOI: 10.1128/IAI.00848-09 en
dc.identifier.issn 0019-9567
dc.identifier.other PURE: 164215
dc.identifier.other PURE UUID: 882e6d20-3403-4bc5-9606-3e72ecf5a841
dc.identifier.other dspace: 2160/7016
dc.identifier.other Ibers_20121112_1204.csv: row: 1030
dc.identifier.other RAD: 1754
dc.identifier.other RAD_Outputs_All_ID_Import_20121105.csv: row: 932
dc.identifier.other Scopus: 77950278221
dc.identifier.other PubMed: 20145100
dc.identifier.other PubMedCentral: PMC2849424
dc.identifier.uri en
dc.description Zhan, B., Perally, S., Brophy, P. M., Xue, J., Goud, G., Liu, S., Deumic, V., de Oliveira, L., Bethony, J., Bottazzi, M. E., Jiang, D., Gillespie, P., Xiao, S., Gupta, R., Loukas, A., Ranjit, N., Lustigman, S., Hotez, P. (2010). Molecular cloning, biochemical characterization, and partial protective immunity of the heme-binding glutathione transferases from the human hookworm Necator americanus. Infection and Immunity, 78 (4), 1552-1563. IMPF: 04.09 en
dc.description.abstract Hookworm glutathione S-transferases (GSTs) are critical for parasite blood feeding and survival and represent potential targets for vaccination. Three cDNAs, each encoding a full-length GST protein from the human hookworm Necator americanus (and designated Na-GST-1, Na-GST-2, and Na-GST-3, respectively) were isolated from cDNA based on their sequence similarity to Ac-GST-1, a GST from the dog hookworm Ancylostoma caninum. The open reading frames of the three N. americanus GSTs each contain 206 amino acids with 51% to 69% sequence identity between each other and Ac-GST-1. Sequence alignment with GSTs from other organisms shows that the three Na-GSTs belong to a nematode-specific nu-class GST family. All three Na-GSTs, when expressed in Pichia pastoris, exhibited low lipid peroxidase and glutathione-conjugating enzymatic activities but high heme-binding capacities, and they may be involved in the detoxification and/or transport of heme. In two separate vaccine trials, recombinant Na-GST-1 formulated with Alhydrogel elicited 32 and 39% reductions in adult hookworm burdens (P < 0.05) following N. americanus larval challenge relative to the results for a group immunized with Alhydrogel alone. In contrast, no protection was observed in vaccine trials with Na-GST-2 or Na-GST-3. On the basis of these and other preclinical data, Na-GST-1 is under possible consideration for further vaccine development. en
dc.format.extent 12 en
dc.language.iso eng
dc.relation.ispartof Infection and Immunity en
dc.rights en
dc.title Molecular Cloning, Biochemical Characterization, and Partial Protective Immunity of the Heme-Binding Glutathione S-Transferases from the Human Hookworm Necator americanus en
dc.type /dk/atira/pure/researchoutput/researchoutputtypes/contributiontojournal/article en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en

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