Characterization of newly established bovine intestinal epithelial cell line 1

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dc.contributor.author Rose, Michael T.
dc.contributor.author Itani, Wataru
dc.contributor.author Miyazawa, K.
dc.contributor.author Yamaguchi, Takahiro
dc.contributor.author Takakura, Ikuro
dc.contributor.author Tanaka, Sachi
dc.contributor.author Kanaya, T.
dc.contributor.author Aso, Hisashi
dc.date.accessioned 2011-06-09T11:42:59Z
dc.date.available 2011-06-09T11:42:59Z
dc.date.issued 2011-06-09
dc.identifier.citation Rose , M T , Itani , W , Miyazawa , K , Yamaguchi , T , Takakura , I , Tanaka , S , Kanaya , T & Aso , H 2011 , ' Characterization of newly established bovine intestinal epithelial cell line 1 ' Unknown Journal , pp. 125-134 . en
dc.identifier.other PURE: 173200
dc.identifier.other dspace: 2160/7033
dc.identifier.uri http://hdl.handle.net/2160/7033
dc.description Miyazawa, K., Kanaya, T., Tanaka, S., Takakura, I., Itani, W., Rose, M. T., Yamaguchi, T., Aso, H. (2010). Characterization of newly established bovine intestinal epithelial cell line 1.   Histochemistry and Cell Biology, 133(1), 125-134 en
dc.description.abstract Membranous epithelial cells (M cells) of the follicle-associated epithelium in Peyer¿s patches have a high capacity for transcytosis of several viruses and microorganisms. Here, we report that we have successfully established a bovine intestinal epithelial cell line (BIE cells) and developed an in vitro M cell model. BIE cells have a cobblestone morphology and microvilli-like structures, and strongly express cell-to-cell junctional proteins and cytokeratin, which is a specific intermediate filament protein of epithelial cells. After co-culture with murine intestinal lymphocytes or treatment with supernatant from bovine PBMC cultured with IL-2, BIE cells acquired the ability of transcytosis. Therefore, BIE cells have typical characteristics of bovine intestinal epithelial cells and also have the ability to differentiate into an M cell like linage. In addition, our results indicate that contact between immune cells and epithelial cells may not be absolutely required for the differentiation of M cells. We think that BIE cells will be useful for studying the transport mechanisms of various pathogens and also the evaluation of drug delivery via M cells. en
dc.format.extent 10 en
dc.language.iso eng
dc.relation.ispartof Unknown Journal en
dc.title Characterization of newly established bovine intestinal epithelial cell line 1 en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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