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dc.contributor.author C. M. en_US
dc.contributor.author J. E. en_US
dc.contributor.author Colin John en_US
dc.contributor.author A. G. S. en_US
dc.contributor.author N. en_US
dc.contributor.author C. en_US
dc.contributor.author Stephen Michael en_US
dc.contributor.author Iain Simon en_US
dc.contributor.author D. E. en_US
dc.contributor.author S. L. en_US
dc.date.accessioned 2011-06-13T11:38:09Z
dc.date.available 2011-06-13T11:38:09Z
dc.date.issued 2011-01 en_US
dc.identifier http://dx.doi.org/10.1016/j.biortech.2010.07.099 en_US
dc.identifier.citation Martel , C M , Parker , J E , Jackson , C J , Warrilow , A G S , Rolley , N , Greig , C , Morris , S M , Donnison , I S , Kelly , D E & Kelly , S L 2011 , ' Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol ' Bioresource Technology , vol 102 , no. 2 , pp. 1503-1508 . , 10.1016/j.biortech.2010.07.099 en_US
dc.identifier.other PURE: 167117 en_US
dc.identifier.other dspace: 2160/7052 en_US
dc.identifier.uri http://hdl.handle.net/2160/7052
dc.description.abstract This study demonstrates use of recombinant yeast to simultaneously saccharify and ferment grass juice (GJ) to bioethanol. A modified Bacillus subtilis levanase gene (sacC) in which the native bacterial signal sequence was replaced with a yeast α-factor domain, was synthesised with yeast codon preferences and transformed into Saccharomyces cerevisiae (strain AH22) using the expression vector pMA91. AH22:psacC transformants secreted sacCp as an active, hyper-glycosylated (>180 kDa) protein allowing them to utilise inulin (β[2-1] linked fructose) and levan (β[2-6] linkages) as growth substrates. The control (AH22:pMA91) strain, transformed with empty plasmid DNA was not able to utilise inulin or levan. When cultured on untreated GJ levels of growth and bioethanol production were significantly higher in experiments with AH22:psacC than with AH22:pMA91. Bioethanol yields from AH22:psacC grown on GJ (32.7[±4] mg mL(-1)) compared closely to those recently achieved (Martel et al., 2010) using enzymatically pre-hydrolysed GJ (36.8[±4] mg mL(-1)). Copyright © 2010 Els en_US
dc.format.extent 6 en_US
dc.relation.ispartof Bioresource Technology en_US
dc.subject Yeast en_US
dc.subject Bioethanol en_US
dc.subject Fructan en_US
dc.subject Perennial ryegrass en_US
dc.title Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol en_US
dc.contributor.pbl Institute of Biological, Environmental and Rural Sciences en_US


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