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dc.contributor.author Martel, C. M.
dc.contributor.author Parker, J. E.
dc.contributor.author Jackson, Colin John
dc.contributor.author Warrilow, A. G. S.
dc.contributor.author Rolley, N.
dc.contributor.author Greig, C.
dc.contributor.author Morris, Stephen Michael
dc.contributor.author Donnison, Iain Simon
dc.contributor.author Kelly, D. E.
dc.contributor.author Kelly, S. L.
dc.date.accessioned 2011-06-13T11:38:09Z
dc.date.available 2011-06-13T11:38:09Z
dc.date.issued 2011-01
dc.identifier.citation Martel , C M , Parker , J E , Jackson , C J , Warrilow , A G S , Rolley , N , Greig , C , Morris , S M , Donnison , I S , Kelly , D E & Kelly , S L 2011 , ' Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol ' Bioresource Technology , vol 102 , no. 2 , pp. 1503-1508 . , 10.1016/j.biortech.2010.07.099 en
dc.identifier.other PURE: 167117
dc.identifier.other dspace: 2160/7052
dc.identifier.uri http://hdl.handle.net/2160/7052
dc.description Martel, C.M., Parker, J.E., Jackson, C.J., Warrilow, A.G.S., Rolley, N., Greig, C., Morris, S.M., Donnison, I.S., Kelly, D.E., Kelly, S.L. (2011). Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol. Bioresource Technology, 102, (2), 1503-1508. IMPF: 04.98 en
dc.description.abstract This study demonstrates use of recombinant yeast to simultaneously saccharify and ferment grass juice (GJ) to bioethanol. A modified Bacillus subtilis levanase gene (sacC) in which the native bacterial signal sequence was replaced with a yeast α-factor domain, was synthesised with yeast codon preferences and transformed into Saccharomyces cerevisiae (strain AH22) using the expression vector pMA91. AH22:psacC transformants secreted sacCp as an active, hyper-glycosylated (>180 kDa) protein allowing them to utilise inulin (β[2-1] linked fructose) and levan (β[2-6] linkages) as growth substrates. The control (AH22:pMA91) strain, transformed with empty plasmid DNA was not able to utilise inulin or levan. When cultured on untreated GJ levels of growth and bioethanol production were significantly higher in experiments with AH22:psacC than with AH22:pMA91. Bioethanol yields from AH22:psacC grown on GJ (32.7[±4] mg mL(-1)) compared closely to those recently achieved (Martel et al., 2010) using enzymatically pre-hydrolysed GJ (36.8[±4] mg mL(-1)). Copyright © 2010 Els en
dc.format.extent 6 en
dc.language.iso eng
dc.relation.ispartof Bioresource Technology en
dc.subject Yeast en
dc.subject Bioethanol en
dc.subject Fructan en
dc.subject Perennial ryegrass en
dc.title Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1016/j.biortech.2010.07.099
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.contributor.institution Institute of Geography & Earth Sciences en
dc.description.status Peer reviewed en


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