Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumption

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dc.contributor.author Lloyd, Amanda Jane
dc.contributor.author Beckmann, Manfred
dc.contributor.author Favé, Gaëlle
dc.contributor.author Mathers, John C.
dc.contributor.author Draper, John
dc.date.accessioned 2011-12-12T12:13:04Z
dc.date.available 2011-12-12T12:13:04Z
dc.date.issued 2011-09-01
dc.identifier.citation Lloyd , A J , Beckmann , M , Favé , G , Mathers , J C & Draper , J 2011 , ' Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumption ' British Journal of Nutrition , vol 106 , no. 6 , pp. 812-824 . en
dc.identifier.other PURE: 174349
dc.identifier.other dspace: 2160/7713
dc.identifier.uri http://hdl.handle.net/2160/7713
dc.description Lloyd, A. J., Beckmann, M., Fave, G., Mathers, J. C., Draper, J. (2011). Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumption. British Journal of Nutrition, 106, (6), 812-824. IMPF: 03.01 en
dc.description.abstract The lack of robust measures of dietary exposure hinders a quantitative understanding of causal relationships between diet and health. Non-targeted metabolite fingerprinting was used to explore the relationships between citrus exposure in free-living human subjects, estimated by a FFQ, and the chemical content of urine. Volunteers (study 1, n 12; study 2, n 11) were classified into high-, medium- and low-frequency citrus consumption groups. Overnight and spot fasting urine samples were obtained after exposure to a standardised citrus-free evening meal. The urine samples were analysed by flow injection electrospray-ionisation MS followed by supervised multivariate data classification analysis to discover discriminatory features associated with the level of citrus exposure. Good separation of high and low citrus consumption classes was achieved. Deeper exploration of high-ranked explanatory mass signals revealed several correlated signals derived from proline betaine. Targeted analysis of the relative levels of proline betaine in both fasting and overnight urine samples demonstrated good correlation with FFQ exposure data. Acute exposure of volunteers to orange juice resulted in the appearance of proline betaine and several biotransformed products in postprandial urine samples. Biomarker validation showed sensitivities of 80·8–92·2 % and specificities of 74·2–94·1 % (false discovery rate-adjusted P values <0·05) for elevated proline betaine in participants who reported high citrus consumption. Proline betaine biotransformation products displayed weaker quantitative relationships with habitual citrus exposure. Targeted screening for the presence of biotransformation products of hesperidin and narirutin, known to be abundant in oranges, revealed that they were relatively poor indicators of citrus exposure. en
dc.format.extent 13 en
dc.language.iso eng
dc.relation.ispartof British Journal of Nutrition en
dc.subject Dietary exposure en
dc.subject Citrus fruits en
dc.subject Metabolomics en
dc.subject Urine en
dc.subject Proline betaine en
dc.subject Biomarkers en
dc.title Proline betaine and its biotransformation products in fasting urine samples are potential biomarkers of habitual citrus fruit consumption en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1017/S0007114511001164
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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