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dc.contributor.author Geyer, Kathrin Karola
dc.contributor.author Rodríguez-López, Carlos Marcelino
dc.contributor.author Hoffmann, Karl Francis
dc.contributor.author Chalmers, Iain Wyllie
dc.contributor.author Munshi, Sabrina Eram
dc.contributor.author Truscott, Martha
dc.contributor.author Heald, James Kevin
dc.contributor.author Wilkinson, Michael J.
dc.contributor.author Hoffmann, Karl Francis
dc.date.accessioned 2011-12-12T14:25:38Z
dc.date.available 2011-12-12T14:25:38Z
dc.date.issued 2011-08-09
dc.identifier.citation Geyer , K K , Rodríguez-López , C M , Hoffmann , K F , Chalmers , I W , Munshi , S E , Truscott , M , Heald , J K , Wilkinson , M J & Hoffmann , K F 2011 , ' Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni ' Nature Communications , vol 2 , 424 . , 10.1038/ncomms1433 en
dc.identifier.issn 2041-1723
dc.identifier.other PURE: 174461
dc.identifier.other dspace: 2160/7720
dc.identifier.uri http://hdl.handle.net/2160/7720
dc.description Geyer, K. K., Rodriguez Lopez, C. M., Chalmers, I. W., Munshi, S. E., Truscott, M., Heald, J., Wilkinson, M. J., Hoffmann, K. F. (2011). Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni. Nature Communications, 2, Article No: 424. K.F.H. and M.J.W. conceived the project; J.H. analysed schistosome m5C by GC–MS; C.M.R.L. assisted in the MSAP analysis of schistosome gDNA and provided valuable direction in the practical studies of schistosome DNA methylation; I.W.C. and M.T. assisted in sodium bisulfite conversions, S.E.M. performed the decitabine/adult worm co-cultivations and K.K.G. conducted all remaining experiments reported herein. K.F.H., M.J.W., C.M.R.L. and K.K.G. drafted and edited the manuscript. K.F.H. directed the project and assembled the manuscript. IMPF: 07.39 RONO: 00 en
dc.description.abstract Similar to other metazoan pathogens, Schistosoma mansoni undergoes transcriptional and developmental regulation during its complex lifecycle and host interactions. DNA methylation as a mechanism to control these processes has, to date, been discounted in this parasite. Here we show the first evidence for cytosine methylation in the S. mansoni genome. Transcriptional coregulation of novel DNA methyltransferase (SmDnmt2) and methyl-CpG-binding domain proteins mirrors the detection of cytosine methylation abundance and implicates the presence of a functional DNA methylation machinery. Genome losses in cytosine methylation upon SmDnmt2 silencing and the identification of a hypermethylated, repetitive intron within a predicted forkhead gene confirm this assertion. Importantly, disruption of egg production and egg maturation by 5-azacytidine establishes an essential role for 5-methylcytosine in this parasite. These findings provide the first functional confirmation for this epigenetic modification in any worm species and link the cytosine methylation machinery to platyhelminth oviposition processes. en
dc.language.iso eng
dc.relation.ispartof Nature Communications en
dc.title Cytosine methylation regulates oviposition in the pathogenic blood fluke Schistosoma mansoni en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.identifier.doi http://dx.doi.org/10.1038/ncomms1433
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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