Technical note: The persistence of microbial-specific DNA sequences through gastric digestion in lambs and its potential use as microbial markers

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dc.contributor.author Belanche, A.
dc.contributor.author Yanez-Ruiz, D. R.
dc.contributor.author Calleja, L.
dc.contributor.author de la Fuente, G.
dc.contributor.author Newbold, C. James
dc.contributor.author Balcells, J.
dc.date.accessioned 2012-01-10T16:34:14Z
dc.date.available 2012-01-10T16:34:14Z
dc.date.issued 2012-01-10
dc.identifier.citation Belanche , A , Yanez-Ruiz , D R , Calleja , L , de la Fuente , G , Newbold , C J & Balcells , J 2012 , ' Technical note: The persistence of microbial-specific DNA sequences through gastric digestion in lambs and its potential use as microbial markers ' Journal of Animal Science , pp. 2812-2816 . en
dc.identifier.other PURE: 183939
dc.identifier.other dspace: 2160/7734
dc.identifier.uri http://hdl.handle.net/2160/7734
dc.description Belanche, A., de la Fuente, G., Yanez-Ruiz, D. R., Newbold, C. J., Calleja, L., Balcells, J. (2011). Technical note: The persistence of microbial-specific DNA sequences through gastric digestion in lambs and its potential use as microbial markers. Journal of Animal Science, 89, (9), 2812-2816. en
dc.description.abstract Two groups of 5 lambs were euthanized at the weaning (T45) and fattening stages (T90) to evaluate the use of microbial ribosomal DNA (rDNA) sequences as potential microbial markers in relation to purine bases (PB) as a conventional marker. Both microbial markers originated similar microbial N concentrations (mg/g of DM), although T45 showed decreased values compared with the T90 group when either PB or rDNA were considered (P = 0.02). The survival of microbial rDNA was determined in 3 digestive sites (omasum, abomasum, and duodenum), but no substantial differences were observed, indicating that rDNA maintains the molecular stability along the sampling sites analyzed. Contrarily PB concentration increased successively along the digestive tract (P <0.05), likely as a consequence of the endogenous PB secretion. Undegraded milk PB may also explain the overestimation of the microbial N concentration (2.8 times greater) using PB than rDNA sequences. Abomasum was the sampling site where the best agreement between PB and rDNA estimations was observed. Protozoal N concentration was irrelevant in T45 animals, although substantial in T90 lambs (18% of microbial N). In conclusion, bacterial 16S and protozoal 18S rDNA sequences may persist through the gastric digestive tract and their utilization as a highly specific microbial marker should not be neglected. en
dc.format.extent 5 en
dc.language.iso eng
dc.relation.ispartof Journal of Animal Science en
dc.title Technical note: The persistence of microbial-specific DNA sequences through gastric digestion in lambs and its potential use as microbial markers en
dc.type Text en
dc.type.publicationtype Article (Journal) en
dc.contributor.institution Institute of Biological, Environmental and Rural Sciences en
dc.description.status Peer reviewed en


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